云母单体颗粒对萎缩性胃炎大鼠胃黏膜癌相关基因蛋白表达的影响

目的:研究云母单体颗粒剂对实验性萎缩性胃炎大鼠癌相关基因表达的调控作用。方法:运用云母高、中、低3种不同剂量的单体颗粒剂,对实验性萎缩性胃炎大鼠进行了分组干预治疗,采用免疫组织化学方法的EnVision系统二步法,观察模型大鼠胃黏膜突变型抑癌基因p53、癌基因p21、抑癌基因p16及细胞凋亡抑制基因bcl-2等基因蛋白表达变化情况。结果:云母3种不同剂量有降低p53,p21表达的倾向,对p16的缺失和bcl-2的高表达有明显调节作用,并能减轻胃黏膜炎症,促进腺体的再生。结论:云母对萎缩性胃炎的治疗和逆转作用可能与对癌相关基因蛋白表达的调控作用有关。

Effect of mica granule on the expression of gene-protein associated with cancer in gastric mucosa tissue of chronic atrophic gastritis rats

Objective: To research the regulative effect of mica monomer granule preparation on the expression of gene associated with cancer in gastric mucosa tissue of experimental chronic atrophic gastritis(CAG) rats.Method: To treat experimental CAG rats using mica monomer granule preparation with three different dosage-high,moderate and low level respectively.To observe the expression changes of mutant antioncogene-p53 gene-protein,oncogene p21,antioncogene p16 and anti-apoptosis gene bcl-2 in gastric mucosa of CAG rats by two-step ways of EnVision system in immunohistochemical method.Result: There was the tendency that mica monomer granule preparation with three different dosage could decrease the expression of p53 as well as p21,and mica had the obvious regulative effects on deletion of p16 and high-expression of bcl-2.It could also alleviate the inflammation of gastric mucosa and promote the regeneration of gland.Conclusion: The treatment and reversion action of mica on chronic atrophic gastritis is probably related with the regulative effect on the expression of gene associated with cancer.