直接荧光法测定槐胺碱的含量

目的:建立一种利用荧光分光光度法快速、灵敏地测定槐胺碱浓度的方法,并应用于苦参碱与氧化苦参碱粗品中槐胺碱含量的测定。方法:采用乙醇 水(2∶8)混合溶剂,在激发波长与发射波长分别为 394 nm和 467nm条件下,用荧光光度计直接测定槐胺碱溶液的荧光强度。结果:此方法在10~200μg·mL^-1线性良好,回归方程为Int= 1.137C+3 875,r=0.998 3,回收率为98%~102%。结论:利用槐胺碱的荧光特性可快速、灵敏地检测槐胺碱含量,此方法不受苦参碱与氧化苦参碱的影响,具有较高的选择性,结果令人满意。

Determination of sophoramine by spectrofluorimetry

OBJECTIVE: The fluorescence property of sophoramine was studied and a spectrofluorimetric method was established to determine the sophoramine content. METHOD: In 20% ethanol solution, with excitation wavelength at 394 nm and emission wavelength at 467 nm, the fluorescence intensity of sophoramine can be detected by the fluorophotometer. RESULT: Sophoramine content can be determined with external standard method by fluorophotometer. The linear relationship between fluorescence intensity and concentration is kept in the range of 10-200 microg x mL(-1). The regression equation is Int = 1.137C + 3.875. The recovery rate is 98%-102%. CONCLUSION: Utilizing the fluorescence character of sophoramine, its content can be determined fast and sensitively. The analysis is not interfered by the existing matrine and oxymatrine. The method has high selectivity and the results is satisfying.