黄芪总黄酮对扑热息痛所致小鼠肝损伤防护作用的研究

目的 :研究黄芪总黄酮 (TFA)对扑热息痛所致肝损伤的防护作用。方法 :用 1%羧甲基纤维素钠 10ml·kg^-1,TFA10 0mg·kg^-1或维生素C (Ascorbicacid ,VC) 1000mg·kg^-1给小鼠灌胃 1h后 ,灌扑热息痛 1000mg·kg^-1,观察小鼠死亡率的变化 ;提前 1h用不同剂量的TFA或VC处理后 ,再灌 400mg·kg^-1的扑热息痛 ,检测血清酶学和肝脏组织学的改变。结果 :给小鼠扑热息痛 1000mg·kg^-1灌胃组 ,24h后可致 80 %小鼠死亡 ;提前 1h用TFA10 0mg·kg^-1或VC灌胃其死亡率可分别下降至 20%和0%。血清转氨酶 (ALT)和病理切片显示当给 400mg·kg^-1扑热息痛灌胃 24h后即可引起严重肝损伤 (ALT升高和肝组织大面积坏死 ,P<0.001)。TFA或VC预防组 (提前1h给药)的肝损伤程度与对照组比较明显减轻 ,其作用强度与药物浓度成正比。结论 :TFA对扑热息痛所致肝损伤有保护作用。

Studies on Protective Effect of Total Flavonoids of Astragalus on Liver Damage Paracetamol-Inducedby

Objective: To study the protective effect of total flavonoids of Astragalus (TFA) on the liver against large doses of paracetamol in mice. Method:After oral administration of TFA or Vitamin C 1h prior to giving large dose of paracetamol in mice, the changes of paracetamol-induced mortality rate, serum enzyme level and liver damage degree were obsezved. Result: Paracetamol produced 80% mortality, within 24 hours of the administration of a dose of 1 000 mg·kg^-1 to the mice. Pre-treatment of the animals with TFA (100 mg·kg^-1) or Vitamin C (1000 mg·kg^-1) reduced the death rate to 20% and0% respectively. There was also a significant rise in the serum enzyme level of alanine transaminase (P<0.001) and the area of liver necrosis (P<0.001), 24h after paracetamol (400mg·kg^-1) treatment. With pre-treatment with either TFA or Vitamin C, there was an obvious dose-dependent decrease in ALT levels and the area of hepatocellular necrosis. Conclusion: TFA has potential protecting effect against the paracetamol-induced hepatic damage.