茉莉酸甲酯对丹参毛状根中丹参酮类成分积累和释放的影响

目的：研究茉莉酸甲酯(methy jasmonat,MJ)对丹参酮类成分的积累和向培养基中的释放的影响。方法：6-7V 悬浮振荡培养ATCC15834 农杆菌诱导的丹参毛状根培养18 d后,液体培养基中加入化学诱导子——茉莉酸甲酯,采用HPLC 的方法,测定MJ处理后不同时间段(2,6,9 d)丹参毛状根中隐丹参酮、丹参酮II_A 的含量以及其在培养基中的含量。结果：毛状根经MJ处理后2,6,9 d隐丹参酮的含量分别达0.039,0.204,0.572 mg·g^-1,分别是同时期未经MJ处理的2.2,8.5,23.8倍；丹参酮IIA的含量达0.251,0.601,1.563 mg·g^-1,分别是同时间期未经MJ处理丹参毛状根的1.9,4.1,6.2倍。结论：MJ能显著促进丹参毛状根中丹参酮类成分的积累并向培养基中释放。

Effects of methyl jasmonat on accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy root

OBJECTIVE: To study the effects of methyl jasmonate (MJ) on the accumulation and release of tanshinones in suspension cultures of Salvia miltiorrhiza hairy roots. METHOD: After 18 day's suspension culture of S. miltiorrhiza hairy roots induced by Agrobacterium rhizogenes ATCC15834, the chemical elicitor--methyl jasmonat was added into 6-7V suspension cultures and at the same time, tanshinones contents (including cryptotanshinone and tanshinone II(A)) on the day 2, 6 and 9, after dealing with MJ, was quantified by HPLC. RESULT: After dealing with MJ on the day 2, 6 and 9, the concentration of cryptotanshinone reached to 0.039, 0.204, 0.571 mg x g(-1) respectively,and tanshinone II(A) reached 0.251, 0.601 and 1.563 mg x g(-1) respectively. After 9 day's treatment by MJ, the maximum increase of cryptotanshinone and tanshinone II(A) were 23.8 fold and 6.2 fold higher than that of the control respectively. CONCLUSION: MJ could stimulate the accumulation of tanshinones in S. miltiorrhiza and have released them into the culture medium.