血竭素高氯酸盐抑制高糖诱导的人肾小球系膜细胞结缔组织生长因子的表达

目的:研究血竭素高氯酸盐抑制高糖诱导的人肾小球系膜细胞(human mesangial cells,HMC)中结缔组织生长因子(connective tissue growth factor,CTGF)的表达,探讨其防治糖尿病肾病(diabetic nephropathy,DN)肾纤维化的机制.方法:将培养的HMC分为低糖组(LG组,5.5 mmol·L~(-1)D-葡萄糖)、高糖组(HG组,25 mmol·L~(-1) D-葡萄糖),每组均设血竭素高氯酸盐浓度7.5 μmol·L~(-1)对照,作用24,48 h后,采用RT-PCR方法和Western blot方法检测CTGF mRNA和蛋白水平的表达.结果:与高糖组比较,低糖组中CTGF mRNA和蛋白表达均显著性减少(P＜0.01),高糖组加血竭素高氯酸盐干预后,CTGF mRNA和蛋白表达显著性减少(P＜0.01).结论:血竭素高氯酸盐可以抑制CTGF的表达,这可能是其防治DN肾纤维化的部分机制.

Dracorhodin perchlorate inhibit high glucose induced connective tissue growth factor formation in human mesangial cells

Objective: To study the effect of dracorhodin perchlorate on high glucose induced connective tissue growth factor (CTGF) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).  Method: The HMC were divided into low glucose group (LG group, 5.5 mmol·L-1 D glucose), high glucose group (HG Group 25 mmol·L-1 D glucose), each group was located with dracorhodin perchlorate (7.5 μmol·L-1) as comparison, and was examined at 24, 48 h. The expression of CTGF mRNA was assessed by semi quantatiue RT PCR, and the expression of CTGF protein was assessed by Western blot.  Result: Compared to HG group, the expression of CTGF mRNA and protein were reduced in LG group after stimulating 24, 48 hours (P<0.01). Compared to HG group, the expression of CTGF mRNA and protein were reduced in HG group by added with dracorhodin perchlorate after stimulating 24, 48 hours (P<0.01).  Conclusion: Dracorhodin perchlorate can inhibit high glucose induced connective tissue growth factor expression in human mesangial cells, and this may be its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).