重楼属植物遗传多样性的RSAP标记

目的:研究重楼属植物DNA指纹图谱,初步探讨RSAP分子标记技术在重楼属植物遗传多样性研究上的应用.方法:采用限制性位点扩增多态性(restriction site amplification polymorphism,RSAP)技术对重楼属8个种进行基因组DNA多态性分析.结果:用10条RSAP引物即选出18对引物组合构建了8个种的DNA指纹图谱.通过遗传距离构建系统进化树,重楼属8个种的进化关系得到了很好的分辨.结论:RSAP标记能够对重楼属植物进行准确的分子鉴定,为重楼属中药材的种类鉴定和种间的分类地位提供分子生物学依据.

Genetic diversity analysis of Paris by RSAP makers

Objective: Studies on DNA fingerprinting of eight species of Paris and application of restriction site amplification polymorphism(RSAP) to the identification of Paris. Method: Sequence-related amplified polymorphism(SRAP)molecular markers were used to detect the genetic diversity of 7 accessions of Paris collected from Tianquan and Baoxing in Sichuan,and one from Lijiang in Yunnan. Result: The DNA fingerprinting of 8 species were generated by 18 primer combination screened from 45 primer combinations. Eight accessions were clustered into 4 groups by genetic distance. Conclusion: Based on molecular biology methods of RSAP analysis, accurate molecular identification could be performed on traditional Chinese medicinal material plants in Paris, and provided molecular evidence for taxonomy and identification of different species in Paris.