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| 肠外翻技术快速发现车前子吸收成分群的研究 | |
| 引用本文： | 谭琴,朱晶晶,王维皓,王智民,崔九成,孔晶晶,祁萌,杨莉.肠外翻技术快速发现车前子吸收成分群的研究[J].中国中药杂志,2011,36(2):150-153. |
| 作者姓名： | 谭琴 朱晶晶 王维皓 王智民 崔九成 孔晶晶 祁萌 杨莉 |
| 作者单位： | 1. 陕西中医学院,陕西,咸阳,712046;中国中医科学院,中药研究所,北京,100700 2. 中国中医科学院,中药研究所,北京,100700 3. 陕西中医学院,陕西,咸阳,712046 4. 上海中医药大学,中药研究所中药标准化教育部重点实验室,上海,201210 |
| 基金项目： | 国家 "重大新药创制" 科技重大专项(2009ZX09308-003,2009ZX09301-005,2009ZX09502-021);2007年中医药行业科技专项(200707009) |
| 摘 要： | 目的:建立快速发现车前子吸收成分群的肠外翻模型,为选择车前子质量控制指标提供依据.方法:采用大鼠肠外翻模型,收集3个浓度车前子提取物给药后不同时间的肠囊液样品,用HPLC和LC-MS"进行检测分析,并计算其有效成分京尼平苷酸的累积吸收量.结果:车前子中6种成分均可进入肠囊,在90 min后能全部检测到;不同浓度车前子中京尼平苷酸在各肠段均为线性吸收,R<'2>均大于0.9,符合零级吸收速率;京尼平苷酸在空肠、回肠中的吸收速率常数(K<,a>)随着车前子提取物浓度增加而增加(P<0.05),符合被动吸收.结论:肠囊对药物成分吸收有选择性,回肠与空肠相比可更多、更快地给出吸收成分信息,选择肠外翻法90 min的样品检测车前子吸收成分比较适宜. |
| 关 键 词： | 车前子 肠外翻技术 HPLC 吸收成分 |
| 收稿时间： | 2010/8/30 0:00:00 |
Study on absorption ingredients of Plantaginis Semen by in vitro everted intestinal sac method |
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| TAN Qin,ZHU Jingjing,WANG Weihao,WANG Zhimin,CUI Jiucheng,KONG Jingjing,QI meng and YANG li.Study on absorption ingredients of Plantaginis Semen by in vitro everted intestinal sac method[J].China Journal of Chinese Materia Medica,2011,36(2):150-153. | |
| Authors: | TAN Qin ZHU Jingjing WANG Weihao WANG Zhimin CUI Jiucheng KONG Jingjing QI meng and YANG li |
| Institution: | Shaanxi University of Chinese Medicine, Shaanxi 712046, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Shaanxi University of Chinese Medicine, Shaanxi 712046, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China;The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China |
| Abstract: | Objective : To establish a method for quick investigating the absorption ingredients of Plantaginis semen and guiding the index selection for its quality control. Method : The absorption of three concentrations of Plantaginis semem was investigated with the in vitro everted intestinal sac (VEIS) model. The intestinal sac contents of jejunum and ileum were collected at different time and geniposidic acid was detected by HPLC and LC-MSn as the representative marker. Result : Six ingredients could be detected. At different concentrations of Plantaginis semen, geniposidic acid tested by VEIS showed that there was a good linear correlation between the drug absorption from the medium across the intestinal epithelium into the sac contents in various intestines section. The absorption of the gut sacs from 0 to 90 min manifested a significant time-dependent manner. The Ka of geniposidic acid in the jejunum and ileum increased along with the raised dosage of the Plantaginis Semen (P<0.05), which indicated a passive absorption manner. Conclusion : This method can be used as a tool to investigate the absorption ingredients of Plantaginis Semen. Comparing with the jejunum, the ileum can provide more absorption information faster. The optimal incubation time in intestinal sac was 90 min. |
| Keywords: | Plantaginis Semen in vitro everted intestinal sac (VEIS) HPLC absorption ingredients |
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