补骨脂对体外黑素细胞黏附和迁移的作用

目的 :研究补骨脂对黑素细胞黏附和迁移的影响。方法 :用补骨脂处理黑素细胞 ,检测其黏附于牛血清纤维结合素和通过微孔滤膜的细胞数量并与生姜和未经药物处理的黑素细胞比较。结果 :补骨脂可显著促进黑素细胞黏附于牛血清纤维结合素和通过微孔滤膜并呈剂量依赖关系 ,但当补骨脂浓度超过 0.2mg·L^-1时这种作用未见增加。补骨脂浓度在 0.01mg·L^-1时 ,黑素细胞黏附并无显著增加 ,但对迁移的影响已可见统计学差异 (P <0.01)。生姜则不具有这样的作用。结论 :在体外 ,补骨脂能明显促进黑素细胞的黏附和迁移 ,这至少可以部分解释应用补骨脂治疗白癜风是有效的。

Effects of MSF on melanocyte adhesion and migration in vitro

Objective:To investigate the Malytea Scurfpea fruit (MSF) on melanocyte adhesion and migration. Method:Human epidermal melanocytes were treated with MSF and Ginger respectively,then adhesion to bovine serum fibronectin coated culture dishes was checked. Control and treated cells were also examined for migration into micropore filters coated with the same protein. Result:Compared with control, MSF treated melanocytes were obviously easier to adhere to the dishes and move into the filters in a dose dependent manner. When the dose of MSF was 200 mg·L^-1 , it could not reincrease melanocyte adhesion and migration. At 10 mg·L^-1 , under every other concentrations of MSF, there was no marked difference among MSF treated, Ginger treated and untreated melanocytes ( P <0.05) when adhesion test were studied. But to migration, even at 10 mg·L ^-1 MSF, there was obvious increased migration compared with MSF untreated or Ginger treated melanocytes ( P <0.01). Conclusion:MSF has effect on melanocyte adhesion and migration,which can explain, in part, the capacity of MSF to modulate melanocyte function in vitiligo lesions.