代谢组学评价黄连对大鼠药理作用的初步研究

目的:利用HPLC-MS/MS的代谢组学分析方法研究黄连给药大鼠尿液的代谢物变化,寻找可能的生物标记物.方法:SD大鼠灌胃给予7 g·kg~(-1)黄连水提液,连续30 d,采集尿样样品,进行HPLC-MS/MS分析,利用PCA分析数据,从中找出生物标记物.结果:给药22 d,黄连组与对照组大鼠尿样代谢组有较大差异,发现了169个生物标记物,其中可能的化合物为草酰乙酸、苹果酸、2-酮戊二酸、去甲肾上腺素、花生四烯酸、5-羟吲哚乙酸等物质,这与黄连抗炎、阻止儿茶酚胺的生物合成、抑制中枢神经、降低能量代谢的药理作用相一致.结论:代谢组学可用于评价药物的药理作用.

Study of metabonomics on pharmacological action appraisalRhizoma Coptidis in rats

Objective: HPLC-MS/MS-based metabonomics method was used to find the possible biomarker of Rhizoma Coptidis in rat urine. Method: Sprague-Dawley rats were successively administrated 7 g·kg^-1 aqueous extract of Rhizoma Coptidis for 30 days, urine were collected by metabolism cages and detected by using the HPLC-MS-MS.All dates were analyzed by the principal component analysis (PCA) through using the SIMCA-P 10.0 software. Result: The PCA demonstrated that the metabolome between treated group and control group had difference in rat urine sample after of 22 days administrated, for treated group 169 kinds of biomarkers were found including oxalacetic acid, malic acid, 2-ketoglutaric acid, NE, arachidonic acid, 5-HIAA and other compounds, the result was consistent with pharmacological effects of R.coptidis, such as antiinflammatory, inhibiting biosynthesis of CA biosynthesis, anti-central nerve and energy metabolism inhibition. Conclusion: Metabonomics may be available in pharmacological action evaluation of drugs.