丹参酮对培养PC12细胞损伤模型的保护作用

目的 :探讨丹参酮对PC12细胞缺血样损伤模型的保护作用。方法 :采用组织培养法 ,以肾上腺嗜铬瘤克隆化细胞株PC12细胞为材料 ,制备缺糖损伤、缺氧损伤、自由基损伤、咖啡因损伤、一氧化氮损伤及谷氨酸毒性损伤模型。结果 :形态学检查发现丹参酮对六种脑缺血样损伤模型中的PC12细胞具有明显保护作用 ,MTT染色和LDH活性测定提示丹参酮可显著提高损伤模型中PC12细胞的存活数 ,降低细胞损伤程度。其中以对缺氧损伤、咖啡因损伤的保护作用尤为显著。结论 :丹参酮对上述六种PC12细胞缺血损伤均有显著的保护作用 ,但其保护作用可能主要与抑制细胞损伤初期病变进程及咖啡因所致细胞钙超载有关。

Protective Effect of Tanshinone on Injuried Cultured PC12 Cellsin Vitro

OBJECTIVE: To investigate the protective effects of tanshinone on ischemia-like injury models. METHOD: Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and glutamic acid injury were used to assay the anti-ischemic roles of tanshinone in cultured PC12 cells, by using morphological examination, MTT staining and LDH measurement. RESULT: It was found that 3. 125-200 microliters.ml-1 tanshinone possessed obvious protective effects on PC12 cells from six ischemia-like injury models. Tanshinone could increase the number of life cells and decrease LDH activity significantly, particularly in hypoxia and caffeine injured model. CONCLUSION: Tanshinone protected PC12 cells from all injury models effectively in vitro. Its action may mainly focus on inhibiting development of the primary period of ischemia injury and calcium overloading injury.