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中药鉴定学新技术新方法研究进展
引用本文:陈士林,郭宝林,张贵君,严铸云,罗光明,孙素琴,吴和珍,黄林芳,庞晓慧,陈建波.中药鉴定学新技术新方法研究进展[J].中国中药杂志,2012,37(8):1043-1055.
作者姓名:陈士林  郭宝林  张贵君  严铸云  罗光明  孙素琴  吴和珍  黄林芳  庞晓慧  陈建波
作者单位:1. 中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室,北京,100193
2. 北京中医药大学中药学院,北京,100102
3. 成都中医药大学药学院,四川成都,611137
4. 江西中医学院,江西南昌,330004
5. 清华大学化学系,北京,100084
6. 湖北中医药大学,湖北武汉,430065
基金项目:国家自然科学基金重点项目(81130069);卫生行业科研专项项目(200802043)
摘    要:作者对近20年中药鉴定学研究和应用的新技术、新方法进行了综述,介绍和评述了每种技术或方法的原理、特点、应用实例及发展方向,探讨了现有新技术在鉴定中的客观性和准确性。DNA条形码技术鉴定能力较强,在鉴定没有背景信息的中药样品及在方法通用性和可数字化方面具有优势,应用前景广阔。光谱鉴定具有指纹特征性,在中药材及饮片的质量管理和鉴定中具有一定的实用性。显微鉴定新技术、色谱鉴定、特定引物PCR标记鉴定、生物效应鉴定、DNA芯片以及仿生技术等其他技术在特定中药鉴定中将发挥重要作用。

关 键 词:中药鉴定  分子鉴定  化学鉴定  性状鉴定  显微鉴定
收稿时间:2012/1/16 0:00:00

Advances of studies on new technology and method for identifying traditional Chinese medicinal materials
CHEN Shilin,GUO Baolin,ZHANG Guijun,YAN Zhuyun,LUO Guangming,SUN Suqin,WU Hezhen,HUANG Linfang,PANG Xiaohui and CHEN Jianbo.Advances of studies on new technology and method for identifying traditional Chinese medicinal materials[J].China Journal of Chinese Materia Medica,2012,37(8):1043-1055.
Authors:CHEN Shilin  GUO Baolin  ZHANG Guijun  YAN Zhuyun  LUO Guangming  SUN Suqin  WU Hezhen  HUANG Linfang  PANG Xiaohui and CHEN Jianbo
Institution:The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Beijing University of Traditional Chinese Medicine, Beijing 100102, China;College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Department of Chemistry, Tsinghua University, Beijing 100084, China;Hubei University of Traditional Chinese Medicine, Wuhan 430065, China;The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract:In this review, the authors summarized the new technologies and methods for identifying traditional Chinese medicinal materials, including molecular identification, chemical identification, morphological identification, microscopic identification and identification based on biological effects. The authors introduced the principle, characteristics, application and prospect on each new technology or method and compared their advantages and disadvantages. In general, new methods make the result more objective and accurate. DNA barcoding technique and spectroscopy identification have their owner obvious strongpoint in universality and digitalization. In the near future, the two techniques are promising to be the main trend for identifying traditional Chinese medicinal materials. The identification techniques based on microscopy, liquid chromatography, PCR, biological effects and DNA chip will be indispensable supplements. However, the bionic identification technology is just placed in the developing stage at present.
Keywords:identification of traditional Chinese medicinal materials  molecular identification  chemical identification  morphological identification  microscopic identification
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