舒肝健脑调郁片对卒中后抑郁大鼠模型下丘脑促肾上腺皮质激素释放激素基因表达的影响

目的：探讨舒肝健脑调郁片的抗抑郁机制，为临床应用提供依据。方法：通过强迫游泳不动时间、糖水消耗量等方法评价大鼠模型行为学指标变化、采用透射电镜观察细胞超微结构和RT-PCR技术研究下丘脑促肾上腺皮质激素释放激素(corticotrophin releasing hormone, CRH)基因表达的影响。结果：舒肝健脑调郁片能够减轻应激造成的海马神经元损伤，对抑郁症大鼠的脑损伤有保护作用。舒肝健脑调郁片可使模型大鼠下丘脑CRH基因表达下降，与模型组比较差异有显著性(P＜0.05)。表明舒肝健脑调郁片可通过下调CRHmRNA表达，抑制HPA轴功能亢进，达到抗抑郁作用。

Effect of Shugan Jiannao Tiaoyu tablets (SJTT) on hypothalamiccorticotrophin releasing hormone gene expression in model ratof post-stroke depression

Objective: To study the anti-depressive mechanism of Shugan Jiannao Tiaoyu tablets (SJTT) and provide evidence for clinical application. Method: Evaluate the behavioral index changes of rat model through the methods of motionless-time of swimming by force, and the consumption of sucrose, etc, meanwhile, use transmission electron microscope (TEM) to observe the cell ultrastructure and study the influence of the hypothalamic corticotrophin releasing hormone (HCRH) gene expression with the RT-PCR technique. Result: SJTT could reduce the hippocampal neuronal damage caused by stress, protect the brain injury of the depressive rats. SJTT could down-regulate model rats' HCRH. Compared with the model group (P<0.05), which indicates that SJTT could inhibit the hyperfunction of HPA Axis by down-regulating model rats' HCRH, to achieve the antidepressant effect.