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| 野生与栽培防风中升麻苷和5-O-甲基维斯阿米醇苷的含量测定及指纹图谱分析 | |
| 引用本文： | 马丽杰,杨滨,冯学锋,殷小杰,李化,格小光,朱立革,曹军.野生与栽培防风中升麻苷和5-O-甲基维斯阿米醇苷的含量测定及指纹图谱分析[J].中国中药杂志,2010,35(13):1731-1734. |
| 作者姓名： | 马丽杰 杨滨 冯学锋 殷小杰 李化 格小光 朱立革 曹军 |
| 作者单位： | 1. 中国中医科学院中药研究所,北京,100700;内蒙古医学院药理教研室,内蒙古,呼和浩特,010059 2. 中国中医科学院中药研究所,北京,100700 3. 黑龙江中医药大学黑龙江,哈尔滨,150040 |
| 基金项目： | 科研院所社会公益研究专项 (2005DIA1J172) |
| 摘 要： | 目的:建立并比较野生和栽培品种防风中升麻苷、5-O-甲基维斯阿米醇苷HPLC测定方法和HPLC指纹图谱。方法:含量测定采用岛津C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(40∶60),检测波长254 nm,流速1.0 mL.min-1,柱温30℃。HPLC指纹图谱测定采用资生堂MGⅡC18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,检测波长254 nm,流速1.0 mL.min-1,柱温30℃。结果:升麻苷加样回收率为99.6%,RSD 0.72%(n=6);5-O-甲基维斯阿米醇苷加样回收率为102.6%,RSD 0.88%。野生与栽培品种升麻苷质量分数分别为(4.96±2.59),(3.61±1.82)mg.g-1;5-O-甲基维斯阿米醇苷质量分数分别为(3.91±2.09),(4.37±2.02)mg.g-1。野生与栽培品种升麻苷和5-O-甲基维斯阿米醇苷质量分数无显著性差异。在此条件下所建立的指纹图谱中防风各成分得到有效分离。结论:升麻苷、5-O-甲基维斯阿米醇苷HPLC测定方法简便,结果准确,HPLC指纹图谱分析可作为防风药材质量控制及分类评价的依据。 |
| 关 键 词： | 防风 升麻苷 5-O-甲基维斯阿米醇苷 指纹图谱 |
| 收稿时间： | 2009/11/4 0:00:00 |
Determination of prim-O-glucosylcimifugin and 5-O-methylvisamm-isoide in Saposhnicovia divaricata and HPLC fingerprint analysis |
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| MA Lijie,YANG Bin,FENG Xuefeng,YIN Xiaojie,LI Hu,GE Xiaoguang,ZHU Lige and CAO Jun.Determination of prim-O-glucosylcimifugin and 5-O-methylvisamm-isoide in Saposhnicovia divaricata and HPLC fingerprint analysis[J].China Journal of Chinese Materia Medica,2010,35(13):1731-1734. | |
| Authors: | MA Lijie YANG Bin FENG Xuefeng YIN Xiaojie LI Hu GE Xiaoguang ZHU Lige and CAO Jun |
| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China;Department of Pharmarcology, Inner Mongolia Medical College, Hohhot 010059, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China;Heilongjiang University of Chinese Medicine, Harbin 150040,China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing 100700,China |
| Abstract: | |
| Keywords: | Saposhnicovia divaricata prim-O-glucosylcimifugin 5-O-methylvisammisoide fingerprint |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |