红花注射液对大鼠肾缺血再灌注损伤的疗效及机制研究

目的：探讨红花注射液对大鼠肾缺血再灌注损伤的疗效及机制。方法: 将75只SD大鼠分为正常对照组、缺血再灌注组、红花注射液低剂量(62.5 mg·kg^-1)、中剂量(125 mg·kg^-1)和高剂量治疗组(250 mg·kg^-1),建立肾缺血再灌注损伤模型,采用血清肌酐、尿素氮和血尿渗透压值评价肾功能,TUNEL检测肾组织细胞凋亡率,免疫组化检测肾组织caspase-3蛋白表达的变化。结果: 缺血再灌注损伤后,肾功能明显减退,细胞凋亡率和caspase-3表达显著升高(P<0.01)；运用红花注射液治疗后,肾功能显著改善,细胞凋亡率和caspase-3表达显著降低 (P<0.01)。结论: 红花注射液对大鼠肾缺血再灌注损伤所致的肾功能损害具有显著的保护作用,该作用可能与红花抑制细胞凋亡及caspase-3表达有关。

Effect of saffor(Carthamus tinctorius) injection on renal ischemia/reperfusion injury in rats

Objective: To study the effect and mechanism of saffor injection on renal ischemia/reperfusion(I/R) injury in rats.Method: Seventy-five SD rats were randomly divided into five groups(n=15,in each),normal control groups,I/R control groups,low-dose treatment groups,middle-dose treatment groups and high-dose treatment groups.After rat's I/R injury model was established,renal function was assessed by measuring serum creatinine,blood urea nitrogen,urine osmotic pressure and urine osmotic pressure/blood osmotic pressure,the apoptosis rate in I/R renal tissure was measured by TUNEL method and caspase-3 concentration was measured by immunohistochemistry.Result: Reperfusion of the ischemic kidney induced marked renal dysfunction.Saffor injection significantly inhibited the reperfusion-associated increase in apoptosis rate and caspase-3 protein absorbance value.Moreover,the renal dysfunction at all treatment groups was markedly ameliorated by Saffor injection.(P<0.01).Conclusion: The results show that saffor injection significantly reduces the renal dysfunction and injury caused by I/R of the kidney,And the protective effect of Saffor injection may be related to the inhibition of cell apoptosis and caspase-3 gene expression following renal I/R.