| 戊己丸不同配比方对大鼠体外CYP3A1/3A2酶活性的影响 |
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| 引用本文： | 翁小刚,李玉洁,杨庆,王怡薇,刘晓霓,韩晓,张甘霖,隋峰,朱晓新,梁日欣.戊己丸不同配比方对大鼠体外CYP3A1/3A2酶活性的影响[J].中国中药杂志,2010,35(9):1164-1169. |
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| 作者姓名： | 翁小刚 李玉洁 杨庆 王怡薇 刘晓霓 韩晓 张甘霖 隋峰 朱晓新 梁日欣 |
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| 作者单位： | 1. 中国中医科学院,中药研究所,北京,100700
2. 北京中医药大学,北京,100029 |
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| 基金项目： | 国家自然科学基金项目(30930114);国家"十一五"科技支撑计划项目子课题(2006BAI08B04-4);国家自然科学基金重大研究计划项目(90709017);国家中医药管理局中医药行业科研专项(2008070036);中国中医科学院自主选题(ZZ2006102) |
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| 摘 要： | 目的:研究戊己丸不同配比方对大鼠体外肝微粒体CYP3A1/3A2酶活性的影响,从"中药配伍-代谢"关系探讨戊己丸的配伍机制.方法:按L9(34)正交表,戊己丸的配比设计为9个受试复方,以睾丸酮为探针药,HPLC检测戊己丸对大鼠体外肝微粒体CYP3A1/3A2酶活性的影响.结果:黄连、吴茱萸、白芍各提取物及戊己丸1号～9号方抑制CYP3A1/3A2的IC50分别为38.96,871.96,15 519.17,43.17,60.47,276.12,133.40,118.08,88.47,64.36,35.13,39.91mg·L-1;黄连及戊己丸均可显著抑制CYP3A1/3A2酶活性,不同配比戊己丸抑制CYP3A1/3A2酶活性作用不同,有统计学差异;随着黄连在戊己丸方中剂量水平的增高,戊己丸组方抑制CYP3A1/3A2酶活性的能力增强,且戊己丸组方中吴茱萸和白芍配比不同可以影响方中黄连抑制CYP3A1/3A2酶活性的程度.结论:戊己丸配比不同,对CYP3A1/3A2酶活性的抑制作用不同,这种差异可能是不同配比戊己丸药效学、药动学差异的原因所在.
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| 关 键 词： | 戊己丸 CYP3A1/3A2 睾丸酮 HPLC 中药配伍 |
| 收稿时间： | 2009/11/9 0:00:00 |
Effects of Wuji pill compound with different compatibility on cytochromeP450 CYP3A1/3A2 in rat liver microsomes in vitro |
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| WENG Xiaogang,LI Yujie,YANG Qing,WANG Yiwei,LIU Xiaoni,HAN Xiao,ZHANG Ganlin,SUI Feng,ZHU Xiaoxin and LIANG Rixin.Effects of Wuji pill compound with different compatibility on cytochromeP450 CYP3A1/3A2 in rat liver microsomes in vitro[J].China Journal of Chinese Materia Medica,2010,35(9):1164-1169. |
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| Authors: | WENG Xiaogang LI Yujie YANG Qing WANG Yiwei LIU Xiaoni HAN Xiao ZHANG Ganlin SUI Feng ZHU Xiaoxin and LIANG Rixin |
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| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Beijing University of Chinese Medicine, Beijing 100029, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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