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蝉拟青霉多糖对老年大鼠免疫功能的调节作用
引用本文:杨介钻,卓佳,陈柏坤,金丽琴,吕建新,李兰娟.蝉拟青霉多糖对老年大鼠免疫功能的调节作用[J].中国中药杂志,2008,33(3):292-295.
作者姓名:杨介钻  卓佳  陈柏坤  金丽琴  吕建新  李兰娟
基金项目:浙江省教育厅资助项目 , 国家重点基础研究发展计划(973计划)
摘    要:目的:研究蝉拟青霉水提物多糖对老龄大鼠免疫功能的调节作用。方法:以生理盐水处理的10~12周龄SD青年大鼠、18月龄SD老龄大鼠为对照组,实验组以不同剂量蝉拟青霉水提物多糖(50,100,200 mg.kg-1.d-1)处理老龄大鼠3周,观察对照组、实验组大鼠腹腔、肺泡巨噬细胞对葡萄球菌的吞噬作用;以MTT法检测大鼠脾组织细胞分别在刀豆蛋白A(ConA),磷酸脂多糖(LPS)诱导下的淋巴细胞增殖活性;同时检测大鼠脾组织细胞内酸性磷酸酶(ACP),乳酸脱氢酶(LDH),精氨酸酶(ARG)等酶活力,并于透射电镜下观察大鼠脾组织超微结构。结果:与青年组比较,老龄组大鼠巨噬细胞的噬菌能力及脾组织淋巴细胞的增殖反应显著降低(P<0.01),脾细胞内ACP,LDH,ARG等酶活力也显著低下(P<0.01)。各种剂量蝉拟青霉多糖作用3周后老龄大鼠的这些指标均有改善,脾组织超微结构显示线粒体、内质网结构明显且数量增多。结论:蝉拟青霉水提物多糖能提高老龄大鼠腹腔、肺泡巨噬细胞吞噬功能和脾细胞免疫功能及增殖反应能力,使老龄大鼠低下的免疫功能得以改善,可能具有一定的抗衰老作用。

关 键 词:蝉拟青霉  多糖  巨噬细胞  增殖反应  免疫功能
文章编号:1001-5302(2008)03-0292-05
收稿时间:2007-03-10
修稿时间:2007年3月10日

Regulating effects of Paecilomyces cicadae polysaccharides on immunity of aged rats
YANG Jie-zuan ;ZHUO Jia ;CHEN Bo-kun ;JIN Li-qin ;LV Jian-xin ;LI Lan-juan.Regulating effects of Paecilomyces cicadae polysaccharides on immunity of aged rats[J].China Journal of Chinese Materia Medica,2008,33(3):292-295.
Authors:YANG Jie-zuan ;ZHUO Jia ;CHEN Bo-kun ;JIN Li-qin ;LV Jian-xin ;LI Lan-juan
Institution:Department of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Key Laboratory of Infectious Diseases Ministry of Public Health, Hangzhou 310003, China.
Abstract:OBJECTIVE: To investigate the effects of Paecilomyces cicadae polysaccharide (PCPS) on the immunological function of aged rats in vivo. METHOD: The young and old rats were administered with normal saline as control groups, and the rats from test group were sc given 50, 100, 200 mg x kg(-1) x d(-1) dosage of PCPS for 3 weeks. The phagocytizing rate and index of PMphi, AMphi to S. aureas were observed, and the colorimetric MTI was used to analyze the proliferative activity of spleenocytes which had been stimulated with ConA or LPS. We also inspected the ability varing of ACP, LDH, ARG of spleen, and observed the ultramicro structure of spleen under the SEM. RESULT: The phagocytosis of Mphi was lower in aged group than that in young' s group, and the proliferative activity of spleenocytes was lower too. The activities of ACP, LDH, ARG of spleen were extremely decreased (P < 0.01) in aged rats as well. The proliferative activity and phagocytotic rate were both extremely increased in PCPS groups (P < 0.01), and the mitochondrion and endoplasmic reticulum of spleen were accrementition as well (P < 0.01). CONCLUSION: PCPS could enhance the phagocytizing function of PMphi, AMphi of aged rats in vivo, and strengthen the immune function of spleen and its proliferative activity as well. Then the immunity of aged rats could be improved. The PCPS may be an anti-aging agent.
Keywords:Paecilomyces cicadae  polysaccharides  macrophages  proliferative activity  immunity
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