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| GC-FID测定大鼠静脉注射新型醒脑静后龙脑的血药浓度及药动学研究 | |
| 引用本文： | 陆洋,杜守颖,陈晓兰,李鹏跃,翟永松,吴清,李冬雪.GC-FID测定大鼠静脉注射新型醒脑静后龙脑的血药浓度及药动学研究[J].中国中药杂志,2011,36(16):2200-2202. |
| 作者姓名： | 陆洋 杜守颖 陈晓兰 李鹏跃 翟永松 吴清 李冬雪 |
| 作者单位： | 1. 北京中医药大学中药学院,北京,100102 2. 首都医科大学中医药学院,北京,100069 3. 中国生物技术发展中心,北京,100039 |
| 基金项目： | 国家自然科学基金面上项目(81073057);国家"重大新药创制"科技重大专项(2009ZX09502-008);教育部博士点基金(20090013110007);北京中医药大学自主选题项目(JYBZZ-JS021) |
| 摘 要： | 目的:建立大鼠血浆中龙脑浓度的GC-FID测定方法,探讨新型醒脑静静脉注射后龙脑在大鼠体内的药代动力学过程.方法:大鼠以10.00 mg· kg-1(以艾片量计)剂量尾静脉注射新型醒脑静后,0.5,1,3,5,8,12,20,30,45 min眼底采血,分离血浆,十八烷为内标,乙酸乙酯萃取,GC-FID测定血浆中龙脑浓度,以Kinetica软件拟合药动学参数.结果:龙脑血药浓度在1.67～16.67 mg·L-1线性关系良好,r =0.999 6,测得低、中、高浓度萃取回收率分别为(92.81±1.11)％,(85.38±0.86)％,(84.58±0.58)％；日内、日间精密度RSD均小于3.0％.静脉注射新型醒脑静后,龙脑在大鼠体内药动学符合二室开放模型,主要药动学参数为t1/2α=(1.18±0.20) min,t1/2β=(22.27±6.85) min,Cmax(Calc)=(18.76±2.10) mg·L-1,MRT=(23.84±7.67) min-1,AUC =(100.00±15.85) mg·min·L-1.结论:建立的GC-FID适用于龙脑血浆药物含量测定及药代动力学研究,新型醒脑静中龙脑在体内分布迅速,代谢较快. |
| 关 键 词： | GC-FID 醒脑静 龙脑 血药浓度 二室模型 |
| 收稿时间： | 2010/11/13 0:00:00 |
Study on pharmacokinetics of borneol in rats injected with novel-Xingnaojing by GC-FID |
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| LU Yang,DU Shouying,CHEN Xiaolan,LI Pengyue,ZHAI Yongsong,WU Qing and LI Dongxue.Study on pharmacokinetics of borneol in rats injected with novel-Xingnaojing by GC-FID[J].China Journal of Chinese Materia Medica,2011,36(16):2200-2202. | |
| Authors: | LU Yang DU Shouying CHEN Xiaolan LI Pengyue ZHAI Yongsong WU Qing and LI Dongxue |
| Institution: | Beijing University of Chinese Medicine, Beijing 100102, China;Beijing University of Chinese Medicine, Beijing 100102, China;Beijing University of Chinese Medicine, Beijing 100102, China;Beijing University of Chinese Medicine, Beijing 100102, China;Capital Medical University, Beijing 100069, China;Beijing University of Chinese Medicine, Beijing 100102, China;China National Center for Biotechnology Development, Beijing 100039, China |
| Abstract: | Objective: To develop a GC-FID method for the determination of borneol concentration in rat plasma and to investigate the pharmacokinetics after injection of novel-Xingnaojing.Method: Novel-Xingnaojing was injected via by caudal vein injection.The blood samples were collected by posterior orbital venous plexus approach at 0.5,1,3,5,8,12,20,30,45 min.The drug in plasma was extracted with ethyl acetate and then detected by GC-FID,octadecane was used as the internal standard.The pharmacokinetic parameters were... |
| Keywords: | GC-FID Xingnaojing borneol plasma concentration two-compartment model |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |