虎杖野生植株及组织培养物中白藜芦醇和虎杖苷含量的比较

目的:比较白藜芦醇和虎杖苷在不同来源的虎杖材料中含量积累的差异,以期筛选出适宜的培养材料用于进一步的代谢调控研究。方法:采用反相高效液相色谱法对不同样品中白藜芦醇及虎杖苷的含量同时进行检测。结果:在多年生野生植株和室内人工栽培幼苗中,白藜芦醇和虎杖苷均在植物根及根茎部特异性累积,其含量要远远高于叶片和茎段。其中,以室内人工栽培3个月的幼苗中有效成分含量较高,虎杖苷的积累量为1.27%,是野生植株的1.25倍,白藜芦醇含量(0.401%)则接近野生植株水平。从虎杖的各种组织培养物中也可检测到白藜芦醇和虎杖苷的存在,但是二者含量的高低与培养物的生长速度、生理状况及发育时期密切相关。在几种培养物中,以毛状根的培养最具潜力,离体培养30 d后,其干重增长率为8.29,分别是自然根和悬浮细胞的8.4倍和192.8倍,而且还可积累一定量的虎杖苷(0.037%)和白藜芦醇(0.007%)。结论:所建立的分析检测方法,具有快速、简便和准确的特点,尤其适于大批量生物样品的同时测定。在几种不同来源的虎杖材料中,毛状根是适宜大规模培养并可持续收获有效成分的培养物。

Contents comparison of resveratrol and polydatin in the wild Polygonum cuspidatum plant and its tissue cultures

Objective: To compare the contents of resveratrol and polydatin in some materials of Polygonum cuspidatum from various sources,so to screen and obtain the suitable cultures for the following metabolism regulation study.Method: RP-HPLC method was applied to simultaneously assay resveratrol and polydatin in different samples.Result: By the modified methods of extraction and determination,large amount of materials were screened.The results indicated that the contents of resveratrol and polydatin in root and rhizome were evidently higher than those in the leave and stems.The content of polydatin in the seedlings cultured indoor for three months was 1.27% and showed a 1.25-time increse than that in the wild plants,while the content of resveratrol(0.401%) approached that in the wild plants.Both of resveratrol and polydatin could be examined from different tissue cultures of P.cuspidatum,such as the sterile seedlings,callus,suspended cells and hairy roots,and the levels of them were closely related to the growth speed,physiological status and developmental phase.Hairy roots had the highest potentiality in several tested cultures and the increase rate of dry weight was 8.29 when cultured in vitro for 30 days,and showed a 8.4-fold and a 192.8-fold increase compared with those of natural roots and suspended cells,respectively.The content of polydatin in the hairy roots was up to 0.037% and that of resveratrol was 0.007%.Conclusion: The established analysis method is rapid,simple and accurate,especially adapted to the simultaneous determination of resveratrol and polydatin in massive biological samples.Hairy-root cultures have the superiority among the tested materials of P.cuspidatum and are suitable for the large-scale biomass and consistent production of efficient constituents.