染料木黄酮对人卵巢癌裸鼠移植瘤影响的实验研究

目的:探讨植物雌激素染料木黄酮对人卵巢癌HO-8910PM细胞裸鼠移植瘤的影响。方法:体外培养人卵巢癌HO-8910PM细胞,裸鼠皮下接种HO-8910PM细胞建立裸鼠移植瘤动物模型,随机分为对照组和3个染料木黄酮治疗组,治疗组分别给予5,25,50 mg.kg^-1染料木黄酮。治疗4周后,应用流式细胞术(FCM)检测各组裸鼠移植瘤细胞周期时相和凋亡率,免疫组化技术检测凋亡基因bcl-2,Fas,FasL蛋白的表达情况,并通过电镜进行形态学观察。结果:50 mg.kg^-1染料木黄酮治疗组裸鼠移植瘤明显小于对照组,肿瘤细胞G₀-G_l期比例升高明显,S期细胞比例显著降低(P<0.01),移植瘤细胞凋亡率为(15.14±2.27)%,明显高于对照组(3.12±1.12)%(P<0.01);移植瘤细胞bc1-2蛋白表达水平明显下降,而Fas蛋白表达水平升高,与对照组比较,均有显著性差异(P<0.05)。电镜观察50 mg.kg^-1染料木黄酮治疗组可见凋亡细胞明显增多。结论:染料木黄酮通过调节移植瘤细胞周期分布和凋亡基因,抑制卵巢癌细胞增殖,诱导细胞凋亡。

Regulative effect of genistein on xenografted tumor of ovarian carcinoma cell on nude mice

Objective: To investigate the regulative effect of genistein on the apoptosis of the xenografted tumors of human ovarian carcinoma HO-8910PM cell on the nude mice.Method: Human ovarian carcinoma HO-8910PM were cultured in vitro.The models of xenografted tumor were established by the transplantation of human ovarian carcinoma HO-8910PM on nude mice.The nude mice were randomly divided into four groups of three treatment groups(in which genistein were administered ip at 5,25 and 50 mg·kg^-1·d^-1,respectively,for 4 weeks) and one control group,the cell cycle and apoptosis of the xenografted tumors were measured by flow cytometry,the expression of bcl-2,Fas and FasL gene of xenografted tumors were determined by the immunohistochemistry and the morphology of tumor cells was observed by electron microscope.Result: The tumor weights of 50 mg·kg^-1 genistein group were more lighter(P<0.05) compared to those of control group.The tumor cells in G₀-G₁ phase were increased,at saml time the cells in S-phase were decreased(P<0.01)after the treatment of 50 mg·kg^-1 genistein.In addition the apoptosis rate of the cell treated with 50 mg·kg^-1 genistein group was(15.14±2.27)%,which was significantly higher than that in the control group(3.12±1.12)%(P<0.01).The expression of bc1-2 of the xenografted tumors was decreased and the expression of Fas was increased in 50 mg·kg^-1 genistein group,both showing a significant difference from those in control group(P<0.05).The apoptosis of the tumor cells were found more under electron microscopy in 50 mg·kg^-1 genistein group than those in control group.Conclusion: Genistein could significantly inhibite the proliferation and induce the apoptosis of the HO-8910PM cell of xenografted tumors by regulating the cell cycle and apoptoic gene in the nude mice.