4种中药复方对大鼠实验性急性应激行为及下丘脑-垂体-肾上腺轴的影响

目的:观察四逆散、半夏厚朴汤、甘麦大枣汤、桂枝甘草龙骨牡蛎汤4种中药复方对大鼠实验性急性应激行为及下丘脑-垂体-肾上腺轴(HPA)的影响。方法:将大鼠分为7组:正常组、模型组、四逆散组、半夏厚朴汤组、甘麦大枣汤组、桂枝甘草龙骨牡蛎汤组,测试各组大鼠应激行为、放射免疫法测定大鼠下丘脑促肾上腺皮质激素释放激素(CRH)、血浆促肾上腺皮质激素(ACTH)及血清皮质酮(CORT)。结果:四逆散减少旷场实验的爬格次数(P<0.01),降低CRH,CORT的含量(P<0.01,P<0.05);半夏厚朴汤延长尾悬挂试验静止时间(P<0.05),降低CRH含量(P<0.05);甘麦大枣汤延长尾悬挂试验静止时间(P<0.05),减少挣扎次数(P<0.01),降低CRH,ACTH,CORT的含量(P<0.01,P<0.05);桂枝甘草龙骨牡蛎汤减少旷场实验的爬格次数(P<0.01),延长尾悬挂试验静止时间(P<0.05),减少尾悬挂试验挣扎次数(P<0.01),降低CRH,ACTH,CORT的含量(P<0.01,P<0.05)。结论:4种中药复方分别通过理气、化痰、补气、温阳等作用于HPA轴不同环节,改变应激行为方式,从而增强机体应对急性应激的能力。

The effect of four kinds of traditional Chinese herbal compound on the experimental acute stress behaviors and the hypothalamus pituitary-adrenal gland axis

Objective: To study the effects of four kinds of traditional Chinese herbal compound,Sini powder,Banxiahoupu soup,Ganmaidazao soup and Guizhigancaolonggumuli soup,on the experimental acute stress behaviors and the hypothalamus-pituitary-adrenal gland axis.Method: Rats were divide into 7 groups: normal group,model 1 and 2 groups,Sini powder group,Banxiahoupu soup group,Ganmaidazao soup group,and Guizhigancaolonggumuli soup group.The behaviors of the rats and their CRH of the hypothalamus,ACTH of plasma and CORT of serum(by the means of radio-immunity) were detected.Result: Sini powder could reduce the crossing times of open-field test(P<0.01) and the contents of CRH and CORT(P<0.01,P<0.05);Banxiahoupu soup could extent the still time of tail-hanging test(P<0.05) and the content of CRH(P<0.05);Ganmaidazao soup could prolong the still time(P<0.05) and reduce the struggle times(P<0.01) of tail-hanging test and reduce the contents of CRH,ACTH and CORT(P<0.01,P<0.05);Guizhigancaolonggumuli soup could reduce the crossing times of open-field test(P<0.01),extent the still time(P<0.05) and reduce the struggle times(P<0.01) of tail-hanging test of tail-hanging test,also reduce the contents of CRH,ACTH and CORT(P<0.01,P<0.05).Conclusion: The four kinds of traditional Chinese herbal compound can increase the ability of anti stress by affect the different taches of the hypothalamus-pituitary-adrenal gland axle and change the stress behaviors,which are based on their respective functions of regulating Qi,dispersing phlegm,tonifying Qi and warming Yang.