| 高效液相色谱法测定地榆中没食子酸的含量 |
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| 引用本文： | 沙明,曹爱民,王冰,刘春丽,杨松松,陈吉文.高效液相色谱法测定地榆中没食子酸的含量[J].中国中药杂志,1999,24(2):99-100. |
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| 作者姓名： | 沙明 曹爱民 王冰 刘春丽 杨松松 陈吉文 |
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| 作者单位： | 辽宁中医学院 沈阳 110032;辽宁省邮电医院 沈阳 110031 |
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| 摘 要： | 目的：建立测定地榆中没食子酸含量的方法。方法：Ｓｈｉｍ-ｐａｃｋＣＬＣ-ＯＤＳ色谱柱，甲醇０．０２５ｍｏｌ／Ｌ磷酸（１５∶８５）为流动相，检测波长２７０ｎｍ。结果：没食子酸与其它组分的色谱峰达到基线分离，样品平均含量为０．２２％，加样回收率均值为９７．４％，ＲＳＤ为１．３％。结论：为控制中药地榆的内在质量提供了新的指标和方法。
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| 关 键 词： | 地榆 没食子酸 高效液相色谱法 |
| 收稿时间： | 1997/12/1 0:00:00 |
Determination of Gallic Acid in Garden Burnet by HPLC |
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| Sha Ming;Cao Aimin;Wang Bing;Liu Chunli ; Yang Songsong; Chen Jiwen.Determination of Gallic Acid in Garden Burnet by HPLC[J].China Journal of Chinese Materia Medica,1999,24(2):99-100. |
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| Authors: | Sha Ming;Cao Aimin;Wang Bing;Liu Chunli ; Yang Songsong; Chen Jiwen |
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| Institution: | Liaoning College of Traditional Chinese Medicine, Shenyang 110032. |
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| Abstract: | OBJECTIVE: To establish a HPLC method for the determination of gallic acid in garden burnet. METHOD: The sample was extracted with methanol under ultrasonic management for 25 min. Chromatographic conditions: Shimadzu LC-6A with SPD-6AV detector at 270 nm and auto injector SIL-6A, methanol-0.025 mol/L phosphoric acid(15:85) as the mobile phase. Shin-pack CLC-ODS column(6.0 mm x 15 mm). RESULT: Gallic acid in garden burnet was baseline separated and determined. The average content was 0.22%, recovery rate 97.4% and relative standard deviation 1.3%. CONCLUSION: Simple, rapid and accurate, the method provides new indexes for quality control of Garden Burnet. |
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| Keywords: | Garden Burnet gallic acid HPLC |
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