| 基于COI序列的紫河车药材及其混伪品的DNA条形码鉴定研究 |
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| 引用本文： | 陈俊,贾静,徐晓兰,辛天怡,张红印,石林春,姚辉,刘冬,吴珍红.基于COI序列的紫河车药材及其混伪品的DNA条形码鉴定研究[J].中国中药杂志,2014,39(12):2204-2207. |
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| 作者姓名： | 陈俊 贾静 徐晓兰 辛天怡 张红印 石林春 姚辉 刘冬 吴珍红 |
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| 作者单位： | 福建农林大学, 福建 福州 350002;长春中医药大学, 吉林 长春 130117;福建农林大学, 福建 福州 350002;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;长春中医药大学, 吉林 长春 130117;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;长春中医药大学, 吉林 长春 130117;福建农林大学, 福建 福州 350002 |
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| 基金项目： | 天然生物毒国家地方工程实验室建设基金项目 |
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| 摘 要： | 利用COI序列对紫河车及其常见混伪品进行DNA条形码分子鉴定，探究准确、快速鉴定紫河车及其混伪品的方法。该研究共收集6个种41份样品，以COI作为条形码序列，对紫河车的正品及其混伪品提取基因组DNA，通过PCR扩增和双向测序，运用CodonCode Aligner进行序列拼接后，采用 MEGA6.0 软件进行序列比对，分析比较种内、种间序列差异，并构建正品紫河车及其混伪品的NJ树。结果表明紫河车COI序列种内平均K2P距离为0.001，种内最大K2P距离为0.008，紫河车的正品来源人与其混伪品种间存在较多变异位点。由所构建的NJ 树显示紫河车与其混伪品均可明显区分。基于COI序列的DNA条形码技术可以鉴定紫河车及其混伪品，为紫河车的鉴别提供了新的工具。
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| 关 键 词： | 紫河车 COI序列 DNA条形码 鉴定 |
| 收稿时间： | 2014/3/15 0:00:00 |
Identification of Placenta hominis and its adulterants using COI barcode |
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| CHEN Jun,JIA Jing,XU Xiao-lan,XIN Tian-yi,ZHANG Hong-yin,SHI Lin-chun,YAO Hui,LIU Dong and WU Zhen-hong.Identification of Placenta hominis and its adulterants using COI barcode[J].China Journal of Chinese Materia Medica,2014,39(12):2204-2207. |
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| Authors: | CHEN Jun JIA Jing XU Xiao-lan XIN Tian-yi ZHANG Hong-yin SHI Lin-chun YAO Hui LIU Dong and WU Zhen-hong |
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| Institution: | Fujian Agriculture and Forestry University, Fuzhou 350002, China;Changchun University of Chinese Medicine, Changchun 130117, China;Fujian Agriculture and Forestry University, Fuzhou 350002, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Changchun University of Chinese Medicine, Changchun 130117, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Changchun University of Chinese Medicine, Changchun 130117, China;Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | In order to provide a new method for the identification of Placenta hominis, the COI barcode has been employed to identify the P. hominis medicinal materials and its adulterants. Genomic DNA was extracted from the experimental samples. The COI sequences were amplified and sequenced bi-directionally. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. NJ tree was constructed by MEGA6.0 software. COI sequences can be successfully obtained from all experimental samples. The intra-specific variation and inter-specific divergence were calculated. The average intra-specific K2P distance of P. hominis was 0.001 and the maximum intra-specific distance was 0.008. The cluster dendrogram constructed can be seen that the same genus is together, and distinguished from its adulterants. It is concluded that P. hominis and its adulterants can be correctly identified by DNA barcoding method. |
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| Keywords: | Placenta hominis COI DNA barcoding identification |
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