活血化瘀方药血塞通胶囊调控CD117+造血干细胞“生新血”的实验研究

目的： 探讨活血化瘀方药血塞通胶囊调控造血干细胞（hemopoietic stem cell，HSC）“生新血”的机制。 方法： 参照改良Zea-Longa法建立大鼠大脑中动脉脑缺血再灌注损伤模型（middle cerebral artery occlusion，MCAO）。随机分为正常组、模型组、血塞通高、中、低剂量组；各组再随机按1，3，7，14，28 d时间点分为亚组。血塞通胶囊配置成20，40，60 g·L^-1灌胃。正常组和模型组生理盐水灌胃，每天1次，直到实验结束。ELISA法检测不同时间点血液和骨髓中干细胞因子（stem cell factor，SCF）表达情况；流式细胞仪检测外周血和骨髓中CD117的变化。 结果： 模型组外周血和骨髓中CD117⁺HSC和SCF从1 d开始升高，14 d到高峰，其后逐渐降低。血塞通高、中剂量组外周血和骨髓中CD117⁺HSC和SCF在同一时间点上升的趋势明显优于模型组（P<0.05）。 结论： 活血化瘀生新层面之“生新血”是通过调控CD117⁺HSC数量变化实现生新血目的。

Research about formulas for activating blood and resolving stasis Xuesaitong capsule regulate CD117+ hemopoietic stem cell to produce new blood

Objective: To investigate the mechanism that the formulas for activating blood and resolving stasis can regulate hemopoietic stem cell to produce new blood. Method: Rats were established animal model of acute cerebral infarction by referencing Olivette'method. They were randomly divided into model group, the group of the high,middle, low dose of the formulas for activating blood and resolving stasis. Each group and then wasrandomly divided into subgroups by 1, 3, 7, 14, 28 d. Xuesaitong capsule was formulated into 20, 40, 60 g·L^-1 with normal saline.The rats were given gavage drugs once a day until the experient ended, and the model group was administrated by intragastrical perfusion of normal saline. ELISA was used to detect the expression of SCF in peripheral blood and bone marrow among different groups at different time points. Flow cytometry was used to observe the changes of CD117 in blood and bone marrow. Result: The CD117⁺HSC and SCF concentration in peripheral blood and bone marrow of model group were increasing during 1-14 d,there was a peak on the 14th day, then the expression was reducing. CD117⁺HSC and SCF concentration rising trend in the group of the high, middle dose of the formulas for activating blood and resolving stasis was preceded model group(P<0.05). Conclusion: Activating blood and resolving stasis can regulate hemopoietic stem cell to produce new blood, and it is through the regulation of CD117⁺HSC number to achieve the purpose.