| mTOR/Akt/FoxO3信号通路在人参皂苷Rg1抗PC-12细胞OGD损伤的作用 |
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| 引用本文： | 严洁萍,沈浓儿,叶强,宗永辉,方晴霞,吕良忠.mTOR/Akt/FoxO3信号通路在人参皂苷Rg1抗PC-12细胞OGD损伤的作用[J].中国中药杂志,2015,40(8):1554-1559. |
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| 作者姓名： | 严洁萍 沈浓儿 叶强 宗永辉 方晴霞 吕良忠 |
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| 作者单位： | 浙江省人民医院药学部,浙江杭州,310014 |
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| 基金项目： | 浙江省自然科学基金项目(Y2100564);浙江省中医药管理局基金项目(2010ZA004) |
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| 摘 要： | 目的:研究人参皂苷Rg1对PC-12细胞缺氧缺糖(oxygen-glucose deprivation,OGD)损伤的保护作用,并初步探讨与mTOR/Akt调控FoxO3核浆穿梭相关的可能的分子机制.方法:建立OGD损伤PC-12细胞模型,MTT 法检测OGD对PC-12细胞存活率.人参皂苷Rg110,20,40 μmol·L-1预处理PC-12细胞24h,加OGD损伤,MTT 检测细胞存活率,比色法检测乳酸脱氢酶(LDH)释放、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量.Western blot法检测mTOR,p-Aktser473,p-Aktthr308,Akt,p-FoxO3,细胞核、细胞质FoxO3和总FoxO3蛋白表达.结果:OGD作用4～24h显著抑制PC-12细胞增殖,呈时间依赖性.Rg1(20,40 μmol·L-1)预处理24h可显著增加细胞存活率,减少LDH释放,增加SOD活力和降低MDA含量.Rg1可抑制由OGD引起的rmTOR,p-Aktser473表达降低.OGD 6 h可减少FoxO3磷酸化,并促进细胞质FoxO3进入细胞核;预处理Rg1增加FoxO3磷酸化,促进FoxO3进入细胞质.提示Rg1可经由调控mTOR/Akt/FoxO3信号通路抑制PC-12细胞损伤.结论:人参皂苷Rg1可抑制OGD引起PC-12细胞损伤,作用机制可能与激活mTOR/Akt信号通路调控FoxO3核质穿梭密切相关.
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| 关 键 词： | PC-12细胞 人参皂苷Rg1 Akt磷酸化 mTOR FoxO3 缺氧缺糖 |
| 收稿时间： | 9/8/2014 12:00:00 AM |
Protective effect of ginsenoside Rg1 again PC-12 cells in OGD injury through mTOR/Akt/FoxO3 signaling pathway |
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| YAN Jie-ping,SHEN Nong-er,YE Qiang,ZONG Yong-hui,FANG Qing-xia and LV Liang-zhong.Protective effect of ginsenoside Rg1 again PC-12 cells in OGD injury through mTOR/Akt/FoxO3 signaling pathway[J].China Journal of Chinese Materia Medica,2015,40(8):1554-1559. |
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| Authors: | YAN Jie-ping SHEN Nong-er YE Qiang ZONG Yong-hui FANG Qing-xia and LV Liang-zhong |
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| Institution: | Department of Pharmacology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China,Department of Pharmacology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China,Department of Pharmacology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China,Department of Pharmacology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China,Department of Pharmacology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China and Department of Pharmacology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China |
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| Abstract: | |
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| Keywords: | PC-12 cell ginsenoside Rg1 Akt phosphorylation mTOR FoxO3 oxygen-glucose deprivation |
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