氨液水解法用于提高黄芪中黄芪甲苷含量的工艺研究

目的:探讨氨试液水解法用于提高黄芪甲苷含量的最佳工艺条件;比较氨解前后黄芪总皂苷的含量差异。方法:采用正交设计试验对影响氨解程度的主要因素进行考察,以黄芪甲苷含量为指标,用高效液相色谱法测定其含量。采用大孔吸附树脂-比色法测定氨解前后黄芪总皂苷的含量。结果:浓氨试液与药材的配比对氨解程度的影响最为显著。最佳氨解工艺参数为:浓氨试液与药材配比1∶5,氨试液浓度为2%时,于90℃水浴回流2 h,黄芪甲苷含量较原药材提高约16倍。比色法结果显示氨解前后黄芪总皂苷含量无统计学差异。结论:氨解法可显著提高黄芪药材中黄芪甲苷的含量,且氨解过程并未影响黄芪总皂苷的含量。说明该法可使其他类型的皂苷转化为黄芪甲苷,具有较高实用价值,可为工业生产提供参考依据。

Amic solution hydrolysis for improving content of astragaloside Ⅳ in extract of Radix Astragali

OBJECTIVE: To optimize amic solution hydrolysis technique for improving the content of astragaloside IV in extract of Radix Astragali. METHOD: Orthogonal design was adopted to investigate the main factors that influenced hydrolysis. Choosing the content of astragaloside IV as inspecting index which determined by HPLC, total saponins was purified with the macro-reticular resine, and contents of saponins before and after hydrolysis were determined by colorimetry at 544 nm. RESULT: The ratio of amic solution to herb medicine was most notable in the four inspected factors. The optimal technique of hydrolysis was that the ratio was 1:5, concentration of amic solution was 2%, and the time of regurgitant extraction at 90 degrees C was two hours. The content of astragaloside IV was notably higher than before by hydrolysis. Difference wasnt significant between the content of saponins before and after hydrolysis by colorimetry. CONCLUSION: Content of astragaloside IV was obviously higher than that before hydrolysis,but the content of total saponins was not changed before and after hydrolysis. The results showed that the other saponins can be transformed into astragaloside IV by hydrolysis. This method was valuable, which provided basis on its industrialization.