|
|||||
|
|
| 通江百合天然群体遗传多样性的SRAP分析 | |
| 引用本文： | 智丽,滕中华,刘旭,李名扬.通江百合天然群体遗传多样性的SRAP分析[J].中国中药杂志,2011,36(14):1921-1926. |
| 作者姓名： | 智丽 滕中华 刘旭 李名扬 |
| 作者单位： | 1. 西南大学 园艺园林学院 重庆市花卉工程技术研究中心 南方山地园艺学教育部重点实验室,重庆,400715 2. 西南大学 农学与生物科技学院,重庆,400715 3. 重庆市药物种植研究所,重庆,408435 |
| 基金项目： | 国家自然科学基金项目(30872063);中央高校基本科研业务费专项资金项目(XDJK2010C077) |
| 摘 要： | 目的 采用SRAP标记技术对通江百合天然群体遗传多样性进行研究.方法 通过SRAP标记对通江百合10个天然群体100个个体遗传多样性进行分析,利用POPGENE 1.32和NTSYS-pc进行数据分析.结果 选择15对引物共扩增出170个位点,多态位点163个,种水平上多态位点百分率为90.58%,Nei's基因多样度指数H0.2631,Shannon's信息指数I0.3661；遗传分化系数Gst0.3672,遗传距离变化范围为0.2021～0.5749.结论 通江百合物种水平上的遗传多样性较高；居群内遗传多样性大于居群间遗传多样性,变异主要存在于居群内部；聚类结果与地理分布表现出明显相关性. |
| 关 键 词： | 通江百合 SRAP 遗传多样性 |
| 收稿时间： | 2011/3/18 0:00:00 |
Genetic diversity analysis of natural groups of Lilium sargentiae by SRAP markers |
|
| ZHI Li,TENG Zhonghu,LIU Xu and LI Mingyang.Genetic diversity analysis of natural groups of Lilium sargentiae by SRAP markers[J].China Journal of Chinese Materia Medica,2011,36(14):1921-1926. | |
| Authors: | ZHI Li TENG Zhonghu LIU Xu and LI Mingyang |
| Institution: | College of Horticulture and Landscape Architecture, Southwest University, Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China;College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China;Chongqing Institute of Material Medical Planting, Chongqing 408435, China;College of Horticulture and Landscape Architecture, Southwest University, Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Chongqing 400715, China |
| Abstract: | Genetic diversity of Lilium sargentiae was detected in this paper by sequence-related amplified polymorphism (SRAP) marker. One hundred wild samples were collected from 10 places, and 15 SRAP primer combinations were used for determination. NTSYS-pc2.1 and POPGEN1.32 were used for data analysis. The results showed that a total of 170 clear DNA bands were amplified, 163 of which were polymorphic. The proportion of polymorphic loci was 90.58% on the level of species. Nei's (1 973) gene diversity (H) was 0.263 1, Shannon's Information index was 0.366 1, the Gst was 0.367 2, and the genetic distance ranged from 0.202 1 to 0.574 9. All materials could be clustered into four groups by UPGMA. The results demonstrated that the genetic diversity of L. sargentiae was rich on the level of species, and the genetic diversity within populations exceeded among populations. The correlations of genetic diversity and distribution were significant in L. sargentiae. |
| Keywords: | Lilium sargentiae SRAP marker genetic diversity |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |