人工冬虫夏草菌液对博莱霉素致小鼠肺纤维化的保护作用

目的：研究人工冬虫夏草菌液(cordyceps sinensi)对博莱霉素(BLM)致肺纤维化小鼠的保护作用。方法：小鼠随机分为4组,模型组经鼻滴入博莱霉素5 mg·kg^-1建立肺间质纤维化模型,人工冬虫夏草菌液预防治疗组于滴入BLM前48 h予人工冬虫夏草菌液灌胃,人工冬虫夏草菌液治疗组于滴入BLM后第14天给人工冬虫夏草菌液灌胃,并与正常小鼠进行对照。第28天收获其支气管肺泡灌洗液(BALF)和肺组织,右肺行苏木精-伊红(HE)和Masson三色染色,并观察电镜下细胞超微结构的改变,左肺经酸解法检测肺组织羟脯氨酸(HYP)含量,用酶联免疫吸附法(ELISA)检测BALF中转化生长因子-β(transforming growth factor-β1,TGF-β1),干扰素-γ(interferon-γ,IFN-γ),白细胞介素4(interleukin-4,IL-4)的浓度。结果：虫草菌液干预组与模型组比较,小鼠肺系数明显下降(P<005)；肺泡炎、纤维化程度均有改善(P<005),其中人工冬虫夏草菌液防治组改善最明显；肺HYP含量下降(P<005)；BALF中TGF-β,IL-4含量亦明显下降(P<005)；防治组可使IFN-γ含量升高(P<005)。结论：人工冬虫夏草菌液可以在某种程度上减轻BLM诱导的肺泡炎及肺纤维化程度,对肺纤维化有一定预防作用；其作用机制可能是通过抑制TGF-β1在肺组织中的表达,并调整Ⅰ型/Ⅱ型细胞因子平衡达到的。

Effects of cordyceps sinensi on bleomycin-induced pulmonary fibrosis in mice

OBJECTIVE: To study the prevention & treatment effect of cordyceps sinensi on bleomycin-induced pulmonary fibrosis (PF) in mice. METHOD: Rats were divided into 4 groups. In BM group (model), pulmonary interstitial fibrosis model was established through nasal dripping of 5 mg x kg(-1) bleomycin. In PCT group (prevention & treatment with cordyceps sinensi), cordyceps sinensi was orally administered 48 h before bleomycin dripping. In CT group (treatment with cordyceps sinensi), cordyceps sinensi was orally administered at 14 d after bleomycin dripping, and NC group (normal mice, control). At 28 d, bronchoalveolar lavage fluid (BALF) and lung tissue were collected, right lung received HE staining and masson staining, the change in cell micro-structure was observed under electron microscope, the hydroxyproline (HYP) content in lung tissue of left lung was detected through acidolysis method, and TGF-beta1, IFN-gamma and IL-4 concentration in BALF was detected through ELISA. RESULT: Compared with those in BM group, mouse lung coefficient decreased significantly (P < 0.05), alveolitis and fibrosis degree improved significantly in PCT and CT groups (P < 0.05), HYP content in lung decreased (P < 0.05). TGF-beta1 and 1L-4 content in BALF decreased significantly in each drug intervention group, IFN-gamma content increased significantly (P < 0.05). CONCLUSION: The cordyceps sinensi can alleviate bleomycin-induced alveolitis and fibrosis degree in a certain degree. The mechanism of the alleviation is possibly due to its inhibition of TGF-beta1 expression and its regulation of imbalance of the type I and II cytokine in lung tissue.