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| HPD系列大孔吸附树脂预处理方法研究 | |
| 引用本文： | 贾存勤,李阳春,屠鹏飞,张洪全.HPD系列大孔吸附树脂预处理方法研究[J].中国中药杂志,2005,30(18):1425-1427. |
| 作者姓名： | 贾存勤 李阳春 屠鹏飞 张洪全 |
| 作者单位： | 1. 北京大学,药学院,北京,100083;甘肃农业大学,草业学院,甘肃,兰州,730050 2. 甘肃农业大学,草业学院,甘肃,兰州,730050 3. 北京大学,药学院,北京,100083 |
| 摘 要： | 目的:建立HPD系列大孔吸附树脂的预处理方法。方法:采用气相色谱法(GC)和紫外分光光度法比较了2%NaOH对HPD系列大孔吸附树脂预处理的影响。结果:树脂先用2%NaOH浸泡后再用乙醇洗脱,对有机残留物的洗脱效果较好。结论:HPD系列树脂预处理方法为:树脂经2%NaOH浸泡洗脱后,在60℃,用乙醇浸泡、洗脱,洗脱流速为2BV.h-1,乙醇用量为3~4倍柱体积,洗脱液的紫外吸收值为0.2~0.5。预处理的树脂经GC检测,苯未检出,其他有机残留物均低于10mg.L-1,达到药用要求。 |
| 关 键 词： | 气相色谱法 HPD系列大孔吸附树脂 预处理 甲苯 萘 |
| 文章编号： | 1001-5302（2005）18-1425-04 |
| 收稿时间： | 2004-12-10 |
| 修稿时间： | 2004-12-10 |
Study on the pretreatment of the series of HPD macroporous adsorption resin |
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| JIA Cun-qin;LI Yang-chun;TU Peng-fei;ZHANG Hong-quan.Study on the pretreatment of the series of HPD macroporous adsorption resin[J].China Journal of Chinese Materia Medica,2005,30(18):1425-1427. | |
| Authors: | JIA Cun-qin;LI Yang-chun;TU Peng-fei;ZHANG Hong-quan |
| Institution: | School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China. |
| Abstract: | OBJECTIVE: To develop a method for the pretreatment of the series of HPD macroporous adsorption resin (MAR). METHOD: The effect of 2% NaOH to pretreatment of the series of HPD MAR was studied by gas chromatography and UV spectrometery. RESULT: The eluting effect by ethanol was better after the MAR was marinated and eluted by 2% NaOH. CONCLUSION: The pretreatment of the series of HPD MAR was: after marinated and eluted by 2% NaOH, the series of HPD MAR were eluted by ethanol at 2 BV x h(-1) at 60 degrees C for 3-4 bed volumes (BV). At then the UV absorbance of eluate was 0.2-0.5. Benzene was not detected and the other residues less than 10 mg x L(-1) in eluted-MAR by GC. And the MARs match to the standard of medicine. |
| Keywords: | gas chromatography series of HPD macroporous adsorption resin pretreatment toluene naphthalene |
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