基于2D-TLC与HPLC-IT-TOF-MS联用技术的雪菊黄酮部位化学成分分析

应用2D-TLC与HPLC-IT-TOF-MS对雪菊黄酮部位的主要成分进行研究,阐明雪菊黄酮部位的化学组成。采用2D-TLC对雪菊黄酮有效部位进行分离;并采用菲罗门Kinetex Evo C₁₈色谱柱(2.1 mm×100 mm,2.6μm),以甲醇-0.05%甲酸水为流动相梯度洗脱;采用ESI离子源,正离子与负离子模式下采集数据。通过对质谱数据分析,与相关文献对照,共指认了18个化合物,包括3个黄酮,1个黄酮醇,9个二氢黄酮,1个二氢黄酮醇,4个查耳酮。2D-TLC能够充分分离雪菊黄酮类成分,HPLC-IT-TOF-MS可以快速准确地分析雪菊黄酮部位的主要化学成分,研究结果为阐明雪菊的药效物质基础提供实验依据。

Analysis of flavonoids in Coreopsis tinctoria by integrating 2D-TLC and HPLC-IT-TOF-MS

The qualitative analysis of flavonoids in Coreopsis tinctoria was carried out by a combination of 2 D-TLC and HPLC-IT-TOF-MS. The separation was conducted on 2 D-TLC and a Phenomenex Kinetex Evo C18 column(2.1 mm×100 mm, 2.6 μm) with methanol-0.05% aqueous formic acid by gradient elution. Electrospray ionization-(ESI) source was applied and operated in both positive and negative ionization modes. Eighteen flavonoids including three flavonoids, one flavonol, nine flavonones, one flavanonol and four chalcones, were putatively identified from the flavone-enriched fraction of C. tinctoria. 2 D-TLC could separate the flavonoids from C. tinctoria. HPLC-IT-TOF-MS was able to quickly and accurately analyze the flavonoids in C. tinctoria. The results would provide experimental information for the efficacy material basis clarification of C. tinctoria.