| 芪苈强心胶囊中12种成分的UPLC-MS测定 |
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| 引用本文： | 刘颖,欧阳玥,李松,刘敏彦,乔莉,赵韶华.芪苈强心胶囊中12种成分的UPLC-MS测定[J].中国中药杂志,2014,39(10):1822-1825. |
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| 作者姓名： | 刘颖 欧阳玥 李松 刘敏彦 乔莉 赵韶华 |
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| 作者单位： | 黑龙江中医药大学, 黑龙江 哈尔滨 150000;河北以岭医药研究院有限公司, 国家中医药管理局重点研究室(心脑血管络病), 河北 石家庄 050035;河北以岭医药研究院有限公司, 国家中医药管理局重点研究室(心脑血管络病), 河北 石家庄 050035;河北以岭医药研究院有限公司, 国家中医药管理局重点研究室(心脑血管络病), 河北 石家庄 050035;河北以岭医药研究院有限公司, 国家中医药管理局重点研究室(心脑血管络病), 河北 石家庄 050035;河北省中西医结合医药研究院, 河北省络病重点实验室, 河北 石家庄 050035;黑龙江中医药大学, 黑龙江 哈尔滨 150000;河北以岭医药研究院有限公司, 国家中医药管理局重点研究室(心脑血管络病), 河北 石家庄 050035 |
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| 基金项目： | 国家重点基础研究发展计划（973计划）项目（2012CB518606）；国家自然科学基金青年基金项目（81102768） |
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| 摘 要： | 该文建立了芪苈强心胶囊中12种有效成分的超高效液相色谱结合质谱（UPLC-MS）的定量方法。采用Phenomenex UPLC Kinetex C18色谱柱（2.1 mm×100 mm，2.6 μm），以乙腈-0.1%甲酸溶液为流动相梯度洗脱，流速0.4 mL·min-1，柱温40 ℃，进样量5 μL，质谱在负离子模式下采用TOF-MS及TOF-MS/MS方法检测。结果表明，12种有效成分毛蕊异黄酮-7-O-葡萄糖苷、异槲皮苷、柚皮芸香苷、橙皮苷、人参皂苷Re、人参皂苷Rg1、杠柳毒苷、人参皂苷Rf、人参皂苷Rb1、黄芪甲苷、人参皂苷Rd、杠柳苷H1在检测的范围内线性良好，R2均大于0.999 0，方法回收率为98.0%~102%，RSD均小于3.9%。该方法简便快速、准确度高，可用于芪苈强心胶囊的质量控制。
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| 关 键 词： | UPLC-MS 芪苈强心胶囊 含量测定 |
| 收稿时间： | 2014/1/15 0:00:00 |
Determination of twelve active compounds in Qili Qiangxin capsules by UPLC-MS |
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| LIU Ying,OUYANG Yue,LI Song,LIU Min-yan,QIAO Li and ZHAO Shao-hua.Determination of twelve active compounds in Qili Qiangxin capsules by UPLC-MS[J].China Journal of Chinese Materia Medica,2014,39(10):1822-1825. |
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| Authors: | LIU Ying OUYANG Yue LI Song LIU Min-yan QIAO Li and ZHAO Shao-hua |
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| Institution: | Heilongjiang University of Traditional Chinese Medicine, Haerbin 150000, China;Yiling Medical Research Institute of Hebei Province, the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease), Shijiazhuang 050035, China;Yiling Medical Research Institute of Hebei Province, the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease), Shijiazhuang 050035, China;Yiling Medical Research Institute of Hebei Province, the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease), Shijiazhuang 050035, China;Yiling Medical Research Institute of Hebei Province, the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease), Shijiazhuang 050035, China;Department of Collateral Theory, Combination of Traditional Chinese Medicinewith Western Medicine Academy of Hebei, Key Laboratory of Network Disease of Hebei Province, Shijiazhuang 050035, China;Heilongjiang University of Traditional Chinese Medicine, Haerbin 150000, China;Yiling Medical Research Institute of Hebei Province, the State Administration of Traditional Chinese Medicine Key Laboratory(Heart and Cerebral Vesselscollateraldisease), Shijiazhuang 050035, China |
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| Abstract: | |
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| Keywords: | UPLC-MS Qili Qiangxin capsules determination |
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