| 淫羊藿苷促进体外培养大鼠骨髓间 充质干细胞的成骨性分化 |
| |
| 引用本文： | 翟远坤,葛宝丰,马慧萍,明磊国,李志锋,程国政,陈克明. 淫羊藿苷促进体外培养大鼠骨髓间 充质干细胞的成骨性分化[J]. 中国中药杂志, 2010, 35(23): 3219-3222 |
| |
| 作者姓名： | 翟远坤 葛宝丰 马慧萍 明磊国 李志锋 程国政 陈克明 |
| |
| 作者单位： | 1. 兰州军区总医院,骨科研究所,甘肃,兰州,730050
2. 兰州军区总医院,药材科,甘肃,兰州,730050 |
| |
| 摘 要： | 目的:研究淫羊藿苷对大鼠体外培养骨髓间充质干细胞(rat bone marrow stromal cells,rBMSCs)成骨性分化的影响.方法:贴壁筛选法体外培养rBMSCs,采用1×10-5mol·L-1的淫羊藿苷进行药物干预,比较淫羊藿苷组和不加药的对照组之间碱性磷酸酶活性、碱性磷酸酶阳性克隆数(CFU-FALP)及钙化结节数量,提取总RNA,RT Real-time PCR检测bFGF,IGF-1,Osterix(OSX),Runx-2 mRNA的表达情况.结果:1×10-5mol·L-1淫羊藿苷可提高碱性磷酸酶(ALP)活性,增加钙化结节和CFU-FALP数量,促进与成骨性分化相关因子bFGF,IGF-1,OSX,Runx-2 mRNA的表达.结论:淫羊藿苷可显著促进rBMSCs的成骨性分化,提示淫羊藿苷具有开发为抗骨质疏松或促进骨折愈合新药的潜力.
|
| 关 键 词： | 淫羊藿苷 骨髓间充质干细胞 成骨性分化 |
| 收稿时间： | 2010-01-12 |
Icariin promotes osteogenic differentiation of rat bone marrow stromal cells in vitro |
| |
| ZHAI Yuankun,GE Baofeng,MA Huiping,MING Leiguo,LI Zhifeng,CHENG Guozheng and ZHOU Jian. Icariin promotes osteogenic differentiation of rat bone marrow stromal cells in vitro[J]. China Journal of Chinese Materia Medica, 2010, 35(23): 3219-3222 |
| |
| Authors: | ZHAI Yuankun GE Baofeng MA Huiping MING Leiguo LI Zhifeng CHENG Guozheng ZHOU Jian |
| |
| Affiliation: | Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China;Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China;Department of Pharmacy, Lanzhou Gereral Hospital, Lanzhou 730050, China;Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China;Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China;Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China;Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou 730050, China |
| |
| Abstract: | Objective : To investigate the effects of icariin on the osteogenic differentiation of rat bone marrow stromal cells(rBMSCs). Method : rBMSCs were cultured by adherence screening method. Icariin was supplemented into the culture at 1×10-5 mol·L-1. The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, CFU-FALP and mineralized bone modulus were compared between the icariin-supplemented group and the control group. Total RNA was isolated and the gene expression of bFGF, IGF-1, Osterix(OSX) and Runx-2 was investigated by RT Real-time PCR. Result : Icariin significantly improved ALP activity, CFU-FALP amounts and mineralized modulus. It also can enhance the mRNA level of bFGF, IGF-1, Osterix and Runx-2. Conclusion : Icariin enhances the osteogenic differentiation of rBMSCs significantly, which suggested that icariin has the potentiality to be a new drug of anti-osteoporosis or fracture healing. |
| |
| Keywords: | icariin BMSCs osteogenic differentiation |
| 本文献已被 万方数据 等数据库收录！ |
|
