地黄不同品种遗传关系的RAPD分析

目的：揭示地黄不同品种的亲缘关系和遗传特点。方法：采用RAPD技术和群体遗传学的分析方法,对地黄16个栽培品种、2个野生居群共54个个体进行了亲缘关系、遗传多样性和遗传结构分析。结果：邻接法构建的多数一致树将54个地黄样品划分为3个明显的类群；Popgene和AMOVA分析均显示地黄品种间的遗传分化大于品种内；类群II所包含的各品种未表现出明显的遗传分化。结论：长期的无性繁殖为地黄积累了一定的遗传分化,野生地黄与栽培品之间遗传距离较大,但在主要栽培品种中遗传分化不明显,显示遗传背景较为单一。野生地黄作为地黄的育种资源应给予充分重视和研究。

RAPD analysis on genetic relationship among varieties of Rehmannia glutinosa

OBJECTIVE: To define the genetic relationship and other genetic characters of different varieties of Rehmannia glutinosa. METHOD: RAPD reactions were conducted on total 54 samples from 16 varieties and 2 populations of R. glutinosa. The data were analyzed by Phylips, Popgene and Arlequin software. RESULT: The majority rule consensus tree defined the 54 samples to 3 groups. Population genetics analysis and AMOVA showed that the genetic diversity among varieties/populations much greater than that within varieties/populations, but within group II the genetic diversity among varieties was similar to that within varieties. CONCLUSION: Due to the long-time asexual propagation of R. glutinosa, some genetic differentiation has been accumulated and fixed within the species. It was shown that the genetic distance between wild population and cultivated varieties was greater than the genetic distance among cultivated varieties. The wild resource of the plant should be paid more attention and studies.