紫外光谱相似度及其在中药注射液质量控制中的应用

目的:建立一种计算紫外光谱相似度的方法实现对中药注射液质量的控制。方法:用紫外吸收光谱上所有数据点计算样品紫外光谱与多个对照品紫外光谱的相似度并求出平均值,通过该平均值与阈值的比较对样品质量进行评价;将相似度新算法与向量夹角余弦、相关系数等算法进行了比较;同时也将作者提出的紫外相似度质量评价方法与色谱相似度的质量评价方法进行了比较。结果:紫外光谱相似度能敏感反映谱图间的差异,应用于14批香丹注射液的质量评价与实际一致。结论:根据紫外光谱相似度大小可实现对中药注射液的质量控制。

Application of UV similarity in quality control of Chinese medical injection

Objective: To propose an algorithm of the similarity between UV-spectra for controlling the quality of Chinese medical injection.Method: The similarity is calculated base on the data of two UV-spectrum curves,thus the sample quality is evaluated according to the average value of the similarity of the UV-spectra between sample with each of standard samples;the proposed algorithm of the similarity is compared with cosine of vectorial angle and relative coefficient;the proposed method for quality evaluation of traditional Chinese injection is discussed with the method according to the chromatographic fingerprint similarity.Result:The calculated similarity with the proposed method can be sensitive to show the difference between UV-spectra.This proposed method for quality evaluation was applied for assessing quality of 14 batches of Xiangdan injection and the results well coincident with sample quality.Conclusion: The proposed method can be used to evaluate the quality of the Chinese medical injection according to the similarity of two UV-spectrum curves.