| 基因芯片技术筛选人参皂苷Rg1促进人神经干细胞增殖的分子靶点研究 |
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| 引用本文： | 李英博,赵香琴,姜英虹,陈笛,王莎莉.基因芯片技术筛选人参皂苷Rg1促进人神经干细胞增殖的分子靶点研究[J].中国中药杂志,2013,38(16):2701-2705. |
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| 作者姓名： | 李英博 赵香琴 姜英虹 陈笛 王莎莉 |
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| 作者单位： | 重庆医科大学 神经科学研究中心, 重庆市神经生物重点实验室, 重庆 400016;重庆医科大学 神经科学研究中心, 重庆市神经生物重点实验室, 重庆 400016;重庆医科大学 神经科学研究中心, 重庆市神经生物重点实验室, 重庆 400016;重庆医科大学 神经科学研究中心, 重庆市神经生物重点实验室, 重庆 400016;重庆医科大学 神经科学研究中心, 重庆市神经生物重点实验室, 重庆 400016 |
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| 基金项目： | 重庆市自然科学基金项目(cstc2011jjA10031) |
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| 摘 要： | 目的: 采用基因芯片技术筛选出人参皂苷Rg1促进人神经干细胞(neural stem cells, NSCs)增殖的主要分子靶点。方法: 首先通过MTT法筛选出Rg1促进NSCs增殖的最佳作用质量浓度为120 mg·L-1。然后通过基因芯片技术,观察Rg1促其增殖7 d时靶基因表达,通过数据演算筛选出Rg1促进NSCs增殖的最主要的靶基因和信号转导途径。结果: 在Rg1促进NSCs增殖第7天时,获得差异基因440个,其中显著上调的基因266个,显著下调的基因174个;HES1基因和CAMP(环磷酸腺苷)-PKA(蛋白激酶A),PI3K(磷脂酰肌醇-3激酶)-AKT信号传导通路与Rg1促进人NSCs增殖密切相关。结论: 基因芯片筛选出的差异表达基因可能为研究Rg1促进NSCs增殖的分子机制研究提供线索。
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| 关 键 词： | 人参皂苷Rg1 人神经干细胞 增殖 基因芯片 |
| 收稿时间： | 2013/3/21 0:00:00 |
Study on molecular target promoting human neural stem cells of ginsenoside Rg1 by gene chip |
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| LI Ying-bo,ZHAO Xiang-qin,JIANG Ying-hong,CHEN Di and WANG Sha-li.Study on molecular target promoting human neural stem cells of ginsenoside Rg1 by gene chip[J].China Journal of Chinese Materia Medica,2013,38(16):2701-2705. |
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| Authors: | LI Ying-bo ZHAO Xiang-qin JIANG Ying-hong CHEN Di and WANG Sha-li |
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| Institution: | Research Center of Neuroscience, Chongqing Medical University, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China;Research Center of Neuroscience, Chongqing Medical University, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China;Research Center of Neuroscience, Chongqing Medical University, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China;Research Center of Neuroscience, Chongqing Medical University, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China;Research Center of Neuroscience, Chongqing Medical University, Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China |
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| Abstract: | Objective: To screen out main molecular target promoting human neural stem cells (NSCs) of ginsenoside Rg1 by using the gene chip technology. Method: First, MTT assay was adopted to screen out the optimal concentration of Rg1-promoted NSC proliferation (120 mg·L-1). Then, on the 7th day after the Rg1-promoted NSC proliferation, the expression of target genes was observed by the gene chip technology. The most important target gene and signal transduction pathways were screened out through the data calculations. Result: On the 7th day after the Rg1-promoted NSC proliferation, obtained 440 differential genes, 266 significantly up-regulated genes and 174 significantly down-regulated genes. HES1 gene, CAMP (cyclic adenosine monophosphate)-PKA (protein kinase A) and PI3K (phosphatidylinositol 3 kinase)-AKT signal transduction pathways were closely related to the NSC proliferation. Conclusion: The differentially expressed genes screened out by gene chip may provide new clues for studies on molecular mechanism of ginsenoside Rg1-promoted NSCs proliferation. |
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| Keywords: | ginsenoside Rg1 human neural stem cell proliferation gene chip |
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