首页 | 本学科首页   官方微博 | 高级检索  
检索        

药用菊花遗传多样性的RAPD分析
引用本文:徐文斌,郭巧生,王长林.药用菊花遗传多样性的RAPD分析[J].中国中药杂志,2006,31(1):8-8.
作者姓名:徐文斌  郭巧生  王长林
作者单位:南京农业大学,中药材研究所,江苏,南京,210095
基金项目:科技部专项基金;国家自然科学基金;中央级科研院所科技基础性工作专项基金
摘    要:目的:分析不同药用菊花栽培类型的遗传差异。方法:采用2%CTAB法进行药用菊花的DNA提取,并用RAPD技术对22个类型药用菊花种质资源的DNA进行检测,采用NTSYS软件对扩增结果进行统计和聚类。结果:筛选得到的26个引物共得到了233条扩增DNA片断,其中89.7%的扩增条带表现出多态性,每个引物平均可扩增得到8.04条多态性片断。聚类分析结果表明利用RAPD技术可将全部供试材料区分开。结论:药用菊花种质资源在分子水平上确实存在较大差异;药用菊花栽培类型间的差异与环境因素有关,但更大程度上由其遗传因素决定。

关 键 词:菊花  RAPD  遗传多样性  聚类分析
文章编号:1001-5302(2006)01-0018-04
收稿时间:2005-03-10
修稿时间:2005-03-10

RAPD analysis for genetic diversity of Chrysanthemum morifolium
XU Wen-bin;GUO Qiao-sheng;WANG Chang-lin.RAPD analysis for genetic diversity of Chrysanthemum morifolium[J].China Journal of Chinese Materia Medica,2006,31(1):8-8.
Authors:XU Wen-bin;GUO Qiao-sheng;WANG Chang-lin
Institution:Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China
Abstract:Objective: To investigate the genetic diversity of Chrysanthemum morifolium at the level of molecular biology.Method:The total genomic DNA was extracted from medicinal chrysanthemums by 2% CTAB method.And the genetic diversity of 22 C.morifolium accessions was tested by RAPD marks.The NTSYS software was used to analyze the marks.Result: 26 10-mer arbitrary primers were found to acquire polymophic results.A total of 233 bands were amplified,of which 89.7% bands were found to be polymophic.8.04 polymophic bands were amplified by each primer on the average.The results of cluster analysis by using UPGMA method showed that all the tested accessions could be differentiated by RAPD marks.Conclusion: There actually existed much genetic diversity at the molecular level among the germplasm resources of C.morifolium.RAPD marks could be effective tools to construct DNA fingerprintings of C.morifolium.The differences between the tested chrysanthemums are related to the environments.However,it was affected by genetic facters more significantly.
Keywords:Chrysanthemum morifolium  RAPD  genetic diversity  cluster analysis
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号