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| 突托蜡梅叶中挥发油成分及其抑菌活性研究 | |
| 引用本文： | 刘易鑫,颜日明,鲁顺保,张志斌,邹峥嵘,朱笃. 突托蜡梅叶中挥发油成分及其抑菌活性研究[J]. 中国中药杂志, 2011, 36(22): 3149-3154 |
| 作者姓名： | 刘易鑫 颜日明 鲁顺保 张志斌 邹峥嵘 朱笃 |
| 作者单位： | 1. 江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,江西南昌330022;江西省林业科学院竹类研究所,江西南昌330032 2. 江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,江西南昌,330022 3. 江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,江西南昌330022;宜春学院江西省天然药物活性成分研究重点实验室,江西宜春336000 |
| 基金项目： | 国家自然科学基金项目(31060090);江西省自然科学基金项目(2009GZN0102,2010GZN0113);江西省教育厅科技项目(GJJ10395,10702) |
| 摘 要： | 目的:研究突托蜡梅Chimonanthus grammatus 叶中挥发油的化学组成及其抑菌活性.方法:采用气相色谱-质谱联用(GC-MS)技术对水蒸气蒸馏法提取的突托蜡梅叶中挥发油进行成分分析,并采用滤纸片琼脂平板扩散法及肉汤稀释法检测该挥发油对9种常见病原菌的抑菌活性.结果:从挥发油中检出了53种成分,所鉴定成分占挥发油色谱总峰面积的99.99％,其主要成分为(E)-3-(4,8-二甲基-3,7-壬烯基)-呋喃(13.81％)、乙酸冰片酯(12.66％)、1,2,3,6-四甲基-双环[2.2.2]辛-2-烯(7.06％)等；抑菌实验表明突托蜡梅叶中挥发油对受试菌均有不同强度的抑制作用.结论:突托蜡梅叶中挥发油对革兰阴性、阳性菌均具有较强的抑菌活性,抑菌谱较广,且该挥发油含有多种生物活性成分,具有很高的药用价值及开发前景. |
| 关 键 词： | 突托蜡梅 挥发油 GC-MS 抑菌活性 |
| 收稿时间： | 2011-01-25 |
Chemical composition and antibacterial activity of the essential oil from leaves of Chimonanthus grammatus |
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| LIU Yixin,YAN Riming,LU Shunbao,ZHANG Zhibin,ZOU Zhengrou and ZHU Du. Chemical composition and antibacterial activity of the essential oil from leaves of Chimonanthus grammatus[J]. China Journal of Chinese Materia Medica, 2011, 36(22): 3149-3154 | |
| Authors: | LIU Yixin YAN Riming LU Shunbao ZHANG Zhibin ZOU Zhengrou ZHU Du |
| Affiliation: | LIU Yixin1,2,YAN Riming1,LU Shunbao1,ZHANG Zhibin1,ZOU Zhengrou1,ZHU Du1,2(1.Key Lab of Protection and Utilization of Subtropic Plant Resources,College of life sciences,Jiangxi Normal University,Nanchang 330022,China,2.Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province,Yichun University,Yichun 336000,3.Jiangxi Academy of Forestry,Nanchang 33032,China) |
| Abstract: | The chemical composition of essential oil, which from the leaves of Chimonanthus grammatus obtained by hydrodistillation were analyzed by GC-MS, and their possible antibacterial properties were screened. According to the results from GC-MS analysis, fifty-three components comprising 99.99% of the essential oil were identified. The major components of essential oil were 3-(4,8-dimethyl-3,7-nonadienyl)-(E)-furan (13.81%), bornyl acetate (12.66%), and 6,6-dimethyl-3-methylene-bicyclo heptane (7.06%), etc. Antibacterial activity of essential oil was employed by two complementary test systems of disc diffusion and MIC/MBC tests, which showed obviously antibacterial activity against all of the tested bacteria. |
| Keywords: | Chimonanthus grammatus essential oil GC-MS antibacterial activity |
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