| HPLC测定雷公藤总萜片中雷公藤内酯醇的含量 |
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| 引用本文： | 濮存海,何明芳,张晓骞,沈佳.HPLC测定雷公藤总萜片中雷公藤内酯醇的含量[J].中国中药杂志,2005,30(4):266-267. |
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| 作者姓名： | 濮存海 何明芳 张晓骞 沈佳 |
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| 作者单位： | 南京中山制药厂,江苏,南京,210012 |
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| 摘 要： | 目的:建立雷公藤总萜片中雷公藤内酯醇含量的HPLC测定方法。方法:采用Lichrospher CN色谱柱,甲醇 水为流动相,梯度洗脱,检测波长 255 nm。结果:雷公藤内酯醇在 0 742~59 36μg·mL-1线性关系良好,r=0.999 8,平均加样回收率为99.2%(n=5),RSD 1.7%。结论:方法简便快捷,无干扰,重复性好,可用于雷公藤总萜片的质量控制。
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| 关 键 词： | 雷公藤总萜片 雷公藤内酯醇 高效液相色谱 含量测定 |
| 文章编号： | 1001-5302(2005)04-0266-02 |
| 收稿时间： | 2003/1/15 0:00:00 |
Determination of triptolide in tripterygium total terpenoids tablets by HPLC |
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| PU Cun-hai;HE Ming-fang;ZHANAG Xiao-qian;SHEN Jia.Determination of triptolide in tripterygium total terpenoids tablets by HPLC[J].China Journal of Chinese Materia Medica,2005,30(4):266-267. |
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| Authors: | PU Cun-hai;HE Ming-fang;ZHANAG Xiao-qian;SHEN Jia |
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| Institution: | Nanjing Zhongshan Pharmaceutical Factory, Nanjing 210012, China. |
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| Abstract: | OBJECTIVE: To develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets. METHOD: A Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm. RESULT: The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%. CONCLUSION: The method is simple, rapid and accurate and can be used for quality control of the tablets. |
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| Keywords: | tripterygium total terpenoids tablets triptolide HPLC assay |
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