明党参与川明参群体遗传结构及分子鉴定的ISSR分析

目的:明党参和川明参群体遗传遗传多样性、遗传结构及分子鉴定的研究。方法:对7个不同野生群体的明党参和1个栽培群体的川明参进行ISSR分析。利用POPGENE分析软件分析了群体的遗传多样性和遗传结构。结果:共检测了152个位点,总的多态性位点百分率P为90.8％,群体遗传分化值Gst为57.5％,明党参群体水平的遗传多样性(A=1.272;P=27.26％;I=0.132;H=0.087)高于川明参群体(A=1.217;P=21.7％;I=0.103;H=0.067)。结论:明党参的遗传多样性较高,遗传变异主要存在于不同群体间。川明参的遗传多样性低于明党参。聚类分析结果表明,川明参与明党参在DNA水平出现了相当的遗传分化。并通过ISSR分析筛选出了川明参一个特有的分子标记。对明党参和川明参的系统发育关系也进行了探讨。

Analysis of Population Genetic Structure and Molecular Identification of Changium smyrnioides and Chuanminshen violaceum With ISSR Marker

Objective: To assess the population genetic diversity and genetic structure and screen species - specific bands for i-dentification of Changium smyrnioides and Chuanminshen violaceum. Method: Seven wild populations of Changium smyrnioides and one cultivated population of Chuanminshen violaceum were studied by ISSR analysis. The population genetic diversity and population genetic structure were assessed by using POPGENE software. Result: A total of 152 ISSR markers were scored, among which 136 (90. 8%) were polymorphic. The values of Gst tended to be high (mean Gst = 0.575). The level of genetic diversity of Changium smyrnioides (A = 1.272; P = 27. 26 % ; I = 0.132;H = 0.087)was higher than that of Chuanminshen violaceum (A = 1.217;P = 21.7;I = 0.103;H =0.067). Conclusion: The genetic variation of Changium smyrnioides is high and the majority of genetic variation occur among populations. Substantial genetic divergence is shown by cluster analysis (UPGMA) to befound between Changium smyrnioides and Chuanminshen violaceum at DNA level. In addition, one species-specific marker has been obtained in Chuanminshen violaceum . The phylogenetic relationship of two species has also been discussed.