| 凝胶法检测2种中药注射液中内毒素含量 |
| |
| 引用本文： | 高双荣,刘婷,郭静,易艳,郝然,李春英,曹春雨,赵雍,回连强,梁爱华.凝胶法检测2种中药注射液中内毒素含量[J].中国中药杂志,2010,35(11):1405-1409. |
| |
| 作者姓名： | 高双荣 刘婷 郭静 易艳 郝然 李春英 曹春雨 赵雍 回连强 梁爱华 |
| |
| 作者单位： | 中国中医科学院中药研究所,北京,100700 |
| |
| 基金项目： | 国家自然科学基金项目(90709043);科技部国家科技攻关计划项目(2006BAI14B05);国家"重大新药创制"科技重大专项(2009ZX09301-005);中国中医科学院自选课题团队建设(2009ZX09502-001) |
| |
| 摘 要： | 目的:通过特异性及非特异性鲎试剂法对鱼腥草注射液及清开灵注射液的内毒素含量的检测,探讨特异性鲎试剂法检测中药注射剂中内毒素的可行性。方法:采用特异性和非特异性的鲎试剂,对不同批次的鱼腥草注射液、清开灵注射液进行检测。结果:特异性及非特异性的鲎试剂均可检测鱼腥草注射液,其无干扰的最小稀释倍数完全一致;用特异性鲎试剂可以检测清开灵注射液,而用非特异性鲎试剂不能检测。结论:在检测清开灵注射液内毒素含量时,用特异性鲎试剂代替非特异性鲎试剂进行检测是一种较为理想的优化方法。
|
| 关 键 词： | 内毒素检测 鲎试剂 鱼腥草注射液 清开灵注射液 |
| 收稿时间： | 2009/12/31 0:00:00 |
Content detection of bacterial endotoxin in two kinds of injection by gelatin technique |
| |
| GAO Shuangrong,LIU Ting,GUO Jing,YI Yan,HAO Ran,LI Chunying,CAO Chunyu,ZHAO Yong,HUI Lianqiang and LIANG Aihua.Content detection of bacterial endotoxin in two kinds of injection by gelatin technique[J].China Journal of Chinese Materia Medica,2010,35(11):1405-1409. |
| |
| Authors: | GAO Shuangrong LIU Ting GUO Jing YI Yan HAO Ran LI Chunying CAO Chunyu ZHAO Yong HUI Lianqiang and LIANG Aihua |
| |
| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| |
| Abstract: | Objective: To detect content of bacterial endotoxin in Yuxingcao and Qingkailing injections by specific and nonspecific tachypleus amebocyte lysate technique for in order to investigate the feasibility of specific tachypleus amebocyte lysate technique for detecting bacterial endotoxin in traditional Chinese drug injections. Method: Different batches of Yuxingcao and Qingkailing injections were detected by specific and nonspecific tachypleus amebocyte lysate kits. Result: Yuxingcao injection could be detected by specific and nonspecific tachypleus amebocyte lysate technique, Whereas Qingkailing injection could be detected only by specific tachypleus amebocyte lysate. Conclusion: Using specific tachypleus amebocyte lysate as a substitute for nonspecific tachypleus amebocyte lysate is an effective method for detecting content of bacterial endotoxin in Qingkailing injection. |
| |
| Keywords: | detection of bacterial endotoxin tachypleus amebocyte lysate technique Yuxingcao injection Qingkailing injection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
