短柄小连翘中的木脂素类化学成分研究

目的:对短柄小连翘Hypericum petiolulatum的化学成分进行系统的研究.方法:采用硅胶、大孔吸附树脂、Sephadex LH-20柱色谱和制备型高效液相色谱方法进行分离;应用NMR和MS等波谱学方法鉴定化合物的结构.结果:从短柄小连翘乙醇提取物中分离得到9个木脂素类成分,分别鉴定为(-)-(2R,3R)-1-O-feruloy1-8,8'-bisdihydrosiringenin(1),(-)-secoisolariciresinol 4-O-β-D-glucopyranoside(2),isolaricire-sinol-β-4'-O-β-D-glucopy-ranoside(3),5-methoxy-9β-xylopyranosyl-(+)-isolariciresinol(4),(+)-lyoniresinol-2α-O-β-D-xylopyranoside(5),5-methoxy-9-β-xylopyranosyl一(一)-isolariciresinol(6),isolariciresinol 6a-O-β-D-glucoside(7),(+)-lyoniresinol 3α-O-β-D-xylopyranoside(8)和7-methoxy-5-benzofuranpropanol 4'-O-β-D-glucopyranoside(9).结论:化合物1为一新化合物,其余化合物均为首次从该属植物中分离得到.

Ligans from branch of Hypericum petiolulatum

Objective: To investigate the chemical constituents of Hypericum petiolulatum.  Method: The chemical constituents were isolated and purified by column chromatography on silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. Structures of the compounds were identified by NMR and MS spectroscopic methods.  Result: Nine lignans were obtained and their structures were elucidated as (-)-(2R,3R)-1-O-feruloyl-8,8′-bisdihydrosiringenin (1), (-)-secoisolariciresinol 4-O-β-D-glucopyrano-side (2), isolariciresinol-β-4′-O-β-D-glucopyranoside (3), 5-methoxy-9β-xylopyra-nosyl-(+)-isolariciresinol (4), (+)-lyoniresinol 2α-O-β-D-xylopyranoside (5), 5-methoxy-9-β-xylopyranosyl-(-)-isolariciresinol (6), isolariciresinol 6a-O-β-D-gluco-side (7), (+)-lyoniresinol 3α-O-β-D-xylopyranoside (8) and 7-methoxy-5-benzofuranpropanol 4′-O-β-D-glucopyranoside (9).  Conclusion: Coupound 1 was new and compounds 2-9 were obtained from the genus Hypericum for the first time.