| 1株选择性降解茅苍术挥发油内生真菌的筛选与鉴定 |
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| 引用本文： | 李蕾,刘付燕,任承钢,戴传超.1株选择性降解茅苍术挥发油内生真菌的筛选与鉴定[J].中国中药杂志,2012,37(19):2855-2862. |
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| 作者姓名： | 李蕾 刘付燕 任承钢 戴传超 |
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| 作者单位： | 南京师范大学 生命科学学院 江苏省微生物与功能基因组学重点实验室江苏省微生物资源产业化工程技术研究中心,江苏 南京,210046 |
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| 基金项目： | 国家自然科学基金项目(31070443,30970523);国家理科基地人才培养项目(J1103507);江苏高教优势学科建设工程项目;南京市科委工程中心创新能力提升项目(201105058) |
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| 摘 要： | 目的:从茅苍术Atractylodes lancea根中分离内生真菌,筛选能选择性降解转化茅苍术挥发油的菌株,实现内生真菌对茅苍术挥发油主要成分的转化作用.方法:利用微生物体外转化方法,通过气相色谱追踪挥发油变化,筛选出能选择性利用挥发油的内生真菌.通过单因素试验研究碳源、转速、装液量、初始pH和植物组织添加对内生真菌降解的影响,并选取对降解影响较大的因素进行正交试验.结果:筛选到的内生真菌ALG-13可以选择性利用挥发油,改变了挥发油主要成分相对百分比.总体表现为苍术酮和苍术素增加,β-桉叶醇和茅术醇含量减少.经过真菌选择性降解后,挥发油组分更加接近道地茅苍术的挥发油组成.ALG-13基于形态特征、ITS序列系统学分析,确定为生赤壳属真菌Bionectria ochroleuca.最优降解条件为200 r·min-1,pH4.5,250 mL装液量为50 mL、蔗糖为碳源.结论:内生真菌ALG-13可以选择性降解挥发油,使挥发油组分接近道地药材.
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| 关 键 词： | 茅苍术 内生真菌 挥发油 选择性利用 生赤壳属 |
| 收稿时间： | 2012/2/14 0:00:00 |
Screening and identification of an endophytic fungus from Atractylodes lancea which utilizes volatile oil selectively |
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| LI Lei,LIU Fu-yan,REN Cheng-gang and DAI Chuan-chao.Screening and identification of an endophytic fungus from Atractylodes lancea which utilizes volatile oil selectively[J].China Journal of Chinese Materia Medica,2012,37(19):2855-2862. |
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| Authors: | LI Lei LIU Fu-yan REN Cheng-gang and DAI Chuan-chao |
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| Institution: | Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Science, Nanjing Normal University, Nanjing 210046, China;Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Science, Nanjing Normal University, Nanjing 210046, China;Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Science, Nanjing Normal University, Nanjing 210046, China;Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Science, Nanjing Normal University, Nanjing 210046, China |
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| Abstract: | |
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| Keywords: | Atractylodes lancea endophytic fungi volatile oil selectively use Bionectria ochroleuca |
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