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黑骨藤多糖含量测定方法研究
引用本文:孙磊,乔善义,赵毅民.黑骨藤多糖含量测定方法研究[J].中国中药杂志,2009,34(10):1241.
作者姓名:孙磊  乔善义  赵毅民
作者单位:军事医学科学院毒物药物研究所,北京,100850
基金项目:

国家科技支撑计划(2006BA108B03-08)

摘    要:目的:对黑骨藤中的活性多糖进行含量测定方法研究.方法:以精制黑骨藤多糖测得黑骨藤多糖对葡萄糖的换算因子;采用苯酚一硫酸比色法测定黑骨藤粗多糖部位的多糖含量.结果:黑骨藤粗多糖部位的多糖含量为54.68%(RSD3.0%,n=6),平均加样回收率101.7%(RSD 6.0%,n=6).结论:该方法简便,快速,测定结果更接近真实值.

关 键 词:黑骨藤  多糖  含量测定

Studies on content determination of polysaccharides in Periploca forrestii
SUN Lei,Jiao-Shan-Xi-,Diao-Yi-Min.Studies on content determination of polysaccharides in Periploca forrestii[J].China Journal of Chinese Materia Medica,2009,34(10):1241.
Authors:SUN Lei  Jiao-Shan-Xi-  Diao-Yi-Min
Institution:Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China
Abstract:

Objective: To establish a method for content determination of polysaccharides in Periploca forrestii.  Method: The conversion coefficient of P. forrestii. polysaccharide to glucose was obtained by refined polysaccharides, and then the content of crude polysaccharides was determined by sulfuric-phenol method.  Result:  The content of crude polysaccharides in P. forrestii. was 54.68% (RSD 3.0%, n=6), and the average recovery was 101.7% (RSD 6.0%, n=6).  Conclusion: The method was simple, rapid, and accurate.

Keywords:

Periploca forrestii  polysaccharides  content determination

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