| 中药复方小续命汤组分活性评价及抗脑缺血有效成分组制备 |
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| 引用本文： | 王月华,秦海林,贺晓丽,杜冠华.中药复方小续命汤组分活性评价及抗脑缺血有效成分组制备[J].中国中药杂志,2011,36(15):2140-2144. |
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| 作者姓名： | 王月华 秦海林 贺晓丽 杜冠华 |
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| 作者单位： | 中国医学科学院药物研究所,北京,100050 |
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| 基金项目： | 国家自然科学基金重点项目(30630073) |
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| 摘 要： | 目的:在前期的研究基础上,通过化学分析和药理活性分析相结合的方法,制备小续命汤抗脑缺血有效成分组.方法:DPPH法测定样品对自由基的清除作用；MTT法测定细胞存活率,观察样品对过氧化氢损伤、谷氨酸损伤及低氧损伤的保护作用；TBA法测定线粒体脂质过氧化.结果:综合分析多靶点活性,40%乙醇洗脱物和中间层的活性最强.结论:40%乙醇洗脱物和中间层按比例混合为小续命汤抗脑缺血有效成分组.
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| 关 键 词： | 小续命汤 有效成分组 脑缺血 |
| 收稿时间： | 2010/10/19 0:00:00 |
Activity evaluation of components and preparation of effective components group of Xiaoxuming decoction for anti-cerebral ischemic |
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| WANG Yuehu,QIN Hailin,HE Xiaoli and DU Guanhua.Activity evaluation of components and preparation of effective components group of Xiaoxuming decoction for anti-cerebral ischemic[J].China Journal of Chinese Materia Medica,2011,36(15):2140-2144. |
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| Authors: | WANG Yuehu QIN Hailin HE Xiaoli and DU Guanhua |
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| Institution: | Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China |
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| Abstract: | Objective : On basis of preliminary studies, to prepare the effective components group of Xiaoxuming decoction for anti-ischemic by combining chemical analysis with pharmacological activity screening. Method : Free radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay; TBA method was used to determine mitochondrial lipid peroxidation. Result : Comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest. Conclusion : 40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction. |
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| Keywords: | Xiaoxuming decoction effective components group cerebral ischemic |
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