人参皂苷Rg3对肿瘤血管生长调控因子蛋白表达抑制作用的研究

目的探讨中药单体人参皂苷20(R)-Rg₃对肿瘤血管生长调控因子(VEGF,bFGF,MMP2)蛋白表达的抑制作用。方法进行人肺腺癌细胞株A549、人脐静脉血管内皮细胞HUVEC304细胞株培养、10^-6mol·L^-1浓度Rg₃药物干预72h,采用免疫组化、基因芯片技术,检测上述因子蛋白表达阳性率和灰度变化以及A549细胞基因的差异表达情况。结果Rg₃干预后,A549细胞VEGF蛋白阳性表达率显著降低(P=0.03),VEGF,Flt,KDT以及MMP2蛋白阳性表达的强度较对照组均有显著下降(P<0.05),内皮细胞VEGF,Flt和KDT蛋白阳性表达的灰度较对照组均有显著下降(P<0.05)。基因芯片差异性表达显示其中14个基因下调,10个基因上调。结论Rg₃可显著抑制肿瘤细胞和血管内皮细胞的血管生长调控因子蛋白表达,通过不同靶点作用于肿瘤细胞影响肿瘤新生血管的生成。

The inhibition of 20(R)-ginsenoside Rg3 on the expressions of angiogenesis factors proteins in human lung adenocarcinoma cell line A549 and HUVEC304 cell

Objective: To study the effect of 20(R)-ginsenoside Rg₃ on the expressions of angiogenesis factors proteins (VEGF,bFGF,MMP-2) in human lung adenocarcinoma cell line A549 and HUVEC304 cell. Method: The cell lines of A549 and HUVEC304 were cultured with 20(R)- Rg₃.The gray scale and positive rate of VEGF, bFGF, MMP-2 were detected by immunohistochemistry. The differential expressions of genes were studied by DNA microarray. Result: The positive rate of VEGF protein in A549 cell decreased significantly as compared with the control group (P=0.03). The gray scales of VEGF, Flt, KDT proteins in both A549 cell lines and HUVEC 304 cell lines decreased (P=0.05). Gray scale of MMP-2 also decreased in A549 cell lines. The result of differential expressions of genes of A549 cell lines showed that 14 genes were down-regulated and 10 genes were up-regulated. Conclusion: The Chinese materia medica of 20(R)-Rg₃ can inhibit the expression of angiogenesis factors proteins via several target genes in both tumour cell and vascular endothelial cell.