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解郁丸对慢性应激大鼠HPA轴和免疫系统的影响
引用本文:施桂兰,库宝善 姚海燕.解郁丸对慢性应激大鼠HPA轴和免疫系统的影响[J].中国中药杂志,2007,32(15):1551-1554.
作者姓名:施桂兰  库宝善 姚海燕
作者单位:北京大学,医学部,基础医学院,药理系,北京,100083
摘    要:目的:观察解郁丸对慢性应激抑郁大鼠行为,以及HPA轴和免疫系统的影响,探讨其可能的抗抑郁机制。方法:采用慢性不可预见性应激造成大鼠抑郁模型,大鼠随机分为正常对照组,慢性应激模型组,慢性应激模型+解郁丸(53,106,212 mg·kg-1低、中、高3个剂量)组,慢性应激模型+丙咪嗪(10 mg·kg-1)组。运用糖水消耗实验测定动物行为,称量肾上腺和胸腺测定肾上腺指数和胸腺指数,采用ELISA检测血清促肾上腺皮质激素、皮质酮、白介素-1β和肿瘤坏死因子-α的含量。结果:与正常对照组相比,慢性应激大鼠的糖水消耗量下降,肾上腺指数上升,胸腺指数下降,血清促肾上腺皮质激素、皮质酮、白介素-1β和肿瘤坏死因子-α的水平升高,均有显著性差异(P<0.001),提示慢性应激大鼠出现神经-内分泌-免疫功能紊乱。与慢性应激模型组比较,中、高剂量的解郁丸可增加慢性应激大鼠的糖水消耗量(P<0.001),同时降低肾上腺指数(P<0.001),缓解胸腺萎缩(P<0.01),低、中、高剂量的解郁丸可以翻转血清促肾上腺皮质激素、皮质酮、白介素-1β和肿瘤坏死因子-α的水平(P<0.05,P<0.01,P<0.001)。结论:解郁丸的抗抑郁作用可能与逆转慢性应激大鼠HPA轴和免疫功能紊乱有关。

关 键 词:解郁丸  慢性不可预见性应激抑郁模型  HPA轴  免疫功能
文章编号:1001-5302(2007)15-1551-04
收稿时间:2007-01-22
修稿时间:2007-01-22

Effects of Jieyuwan on HPA axis and immune system in chronic stress models in rats
SHI Gui-lan; KU Bao-shan; YAO Hai-yan.Effects of Jieyuwan on HPA axis and immune system in chronic stress models in rats[J].China Journal of Chinese Materia Medica,2007,32(15):1551-1554.
Authors:SHI Gui-lan; KU Bao-shan; YAO Hai-yan
Institution:Department of Pharmacology, School of Basic Medical Science, Peking University, Beijing 100083, China.
Abstract:OBJECTIVE: To observe the effects of jieyuwan on neuroendocrine-immune of chronic unpredictable stress rats, for identifing its mechanism of action. METHOD: Animals were randomly assigned to six groups: one non-stressed (control) group, one stressed group (CUS + vehicle) , three treatment groups (CUS + Jieyuwan 53, 106, 212 mg x kg(-1), respectively), and one group for imipramine (CUS + imipramine 10 mg x kg(-1)). We used the chronic unpredictable stress (CUS) test in rats to investigated the effects of chronic oral administration (21 days) of Jieyuwan and imipramine (ip) on the sucrose consumption, the relative weight of adrenal and thymus, the concentration of serum corticosterone, adrenocorticotropic hormone, TNF-alpha, IL-1beta measured by ELISA. RESULT: Our results showed that Sprague-Dawley rats subjected to CUS exhibited increased adrenal weight, reduction in thymus weight, less sucrose consumption, and synchronized with higher serum corticosterone, adrenocorticotropic hormone, TNF-alpha, IL-1beta concentration. Chronic treatment with Jieyuwan (53, 106, 212 mg x kg(-1), po, 21 days) could improve locomotion of stressed rat sucrose consumption, turn over the high levels of serum corticosterone, adrenocorticotropic hormone, TNF-alpha, IL-1beta concentration. CONCLUSION: The therapeutic actions of jieyuwan, in some degree, may be related with indirect or direct effects on HPA axis and immune system.
Keywords:Jieyuwan  chronic unpredictable stress  HPA axis  immune function
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