| HPLC法测定不同产地和品种石韦中绿原酸和芒果苷的含量 |
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| 引用本文： | 李开通,张艺轩,曹阳,石钺.HPLC法测定不同产地和品种石韦中绿原酸和芒果苷的含量[J].中国中药杂志,2010,35(16):2075-2078. |
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| 作者姓名： | 李开通 张艺轩 曹阳 石钺 |
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| 作者单位： | 中国医学科学院,北京协和医学院,药用植物研究所,北京,100193 |
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| 基金项目： | 《中国药典》2010版一部标准研究(YS-211,YS-212) |
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| 摘 要： | 目的:建立石韦药材及饮片中绿原酸和芒果苷含量的HPLC测定方法。方法:色谱柱为Diamonsil(TM)钻石C18(4.6 mm×250 mm,5μm),流动相为乙睛-0.5%磷酸水溶液梯度洗脱,检测波长320 nm。结果:绿原酸与芒果苷分别在5.2~130,1.2~18 mg.L-1线性关系良好;绿原酸的平均加样回收率97.9%,RSD 1.9%;芒果苷的平均加样回收率99.6%,RSD 2.9%。不同来源药材及饮片间绿原酸和芒果苷含量差异显著。结论:本方法对石韦药材及饮片的质量标准制定奠定了基础,也为鉴别不同品种石韦提供了参考。
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| 关 键 词： | 石韦 绿原酸 芒果苷 高效液相色谱 质量标准 |
| 收稿时间： | 2010/4/19 0:00:00 |
Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC |
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| LI Kaitong,ZHANG Yixuan,CAO Yang and SHI Yue.Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC[J].China Journal of Chinese Materia Medica,2010,35(16):2075-2078. |
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| Authors: | LI Kaitong ZHANG Yixuan CAO Yang and SHI Yue |
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| Institution: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China |
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| Abstract: | |
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| Keywords: | Pyrrosiae Folium chlorogenic acid mangiferin HPLC quality standard |
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