3′-甲氧基葛根素对大鼠脑缺血再灌注损伤保护作用研究

目的:观察3'-甲氧基葛根素对大鼠局灶性脑缺血再灌注损伤的脑梗死体积、自由基变化的影响,探讨3'-甲氧基葛根素对脑缺血损伤的保护作用.方法:采用线栓法诱导大鼠大脑中动脉栓塞建立局灶性脑缺血再灌注模型,分假手术组、模型组、3'-甲氧基葛根素2个剂量组(5,10 mg·kg~(-1)·d~(-1))和尼莫地平组(5 mg·kg~(-1)·d~(-1)).观察行为学的改变,脑梗死体积和测定超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量.结果:3'-甲氧基葛根素具有显著减轻神经功能缺损症状,减少梗死体积,显著提高脑缺血再灌注损伤大鼠脑皮质SOD活力和降低MDA含量,其中10 mg·kg~(-1)的3'-甲氧基葛根素作用尤为显著.结论:3'-甲氧基葛根素对大鼠局灶性脑缺血再灌注损伤具有保护作用.

Protective effect of 3′-methoxy puerarin on cerebral ischemic injury/ reperfusion in rats

Objective: To observe the effect of 3′-methoxy puerarin on cerebral infarction volume and free radical change of focal cerebral ischemia-reperfusion injury in rats and discuss the protective effect of 3′-methoxy puerarin on the cerebral ischemic/reperfusion injury.  Method: The thread method was used to induce middle cerebral artery embolization, to establish the focal cerebral ischemia-reperfusion model. Rats were divided into five groups: the sham and model group, the two-dose group (5, 10 mg·kg-1·d-1) of 3′-methoxy puerarin and nimodipine group (5 mg·kg-1·d-1). The behavior changes and volume of cerebral infarction were observed, and the leves of SOD and the content of MDA were measured.  Result: 3′-methoxy puerarin could significantly improve the symptoms of neurological deficit and reduce the infarct volume, and increased SOD activity and reduced the content of MDA  of cortex in cerebral ischemia-reperfusion injury rat, the action of 10 mg·kg-1 of 3′-methoxy puerarin is more remarkable.  Conclusion: 3′-methoxy puerarin has protective effect on focal cerebral ischemia-reperfusion injury in rat.