| 4种中药单体对人胰腺癌PANC-1细胞移植瘤生长的影响及分子机制探讨 |
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| 引用本文： | 潘会君,聂绪强,刘朵,卞卡.4种中药单体对人胰腺癌PANC-1细胞移植瘤生长的影响及分子机制探讨[J].中国中药杂志,2013,38(2):245-248. |
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| 作者姓名： | 潘会君 聂绪强 刘朵 卞卡 |
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| 作者单位： | 1. 上海中医药大学穆拉德中药现代化研究中心,上海,201203
2. 上海中医药大学穆拉德中药现代化研究中心,上海201203;上海教委高校一氧化氮和炎症医学E-研究院,上海201203 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09311-003); 上海市教委高校一氧化氮与炎症医学E研究院计划项目 (E-04010) |
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| 摘 要： | 目的:研究淫羊藿次苷Ⅱ,知母皂苷AⅢ,甲基莲心碱,红景天苷对PANC-1移植瘤的作用.方法:建立PANC-1皮下移植瘤模型,成瘤后随机分组,治疗组给予中药单体腹腔注射,23 d取瘤称重,部分组织中性福尔马林固定做免疫组化,其余组织液氮速冻,然后转移至-80℃冰箱保存做基因蛋白分析.免疫组化Ki67观察肿瘤增殖情况,RT-PCR检测瘤组织VEGF mRNA表达情况,Western blotting检测瘤组织VEGF蛋白表达.结果:与模型组比较,知母皂苷AⅢ(1.0 mg·kg-)能抑制肿瘤生长(P＜0.05),能降低VEGF mRNA(P＜0.05)和VEGF蛋白表达(P＜0.05),活化Caspase-3蛋白.淫羊藿次苷Ⅱ,甲基莲心碱,红景天苷的抗肿瘤作用不显著.结论:知母皂苷AⅢ其抗肿瘤作用与抑制VEGF及促进肿瘤凋亡有关.
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| 关 键 词： | 中药单体 血管内皮生长因子 凋亡 |
| 收稿时间： | 2012/6/27 0:00:00 |
Effects of four kinds of Chinese medicine monomer on growth of PANC-1 xenograft tumor and studying of molecular mechanism |
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| PAN Hui-jun,NIE Xu-qiang,LIU Duo and BIAN Ka.Effects of four kinds of Chinese medicine monomer on growth of PANC-1 xenograft tumor and studying of molecular mechanism[J].China Journal of Chinese Materia Medica,2013,38(2):245-248. |
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| Authors: | PAN Hui-jun NIE Xu-qiang LIU Duo and BIAN Ka |
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| Institution: | Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Division of Nitric Oxide and Inflammatory Medicine, E-Institute of Shanghai Universities, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | Chinese medicine monomer vascular endothelial growth factor apoptosis |
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