近红外光谱法测定不同厂家银黄颗粒中黄芩苷含量

目的:采用近红外光谱法对不同厂家银黄颗粒中黄芩苷含量进行快速测定.方法:以HPLC分析值作为参照,采用近红外漫反射光谱技术采集银黄颗粒的近红外光谱,结合偏最小二乘法(PLS)建立黄芩苷含量的快速测定方法.结果:建立的黄芩苷校正模型相关系数(R~2)、内部交叉验证均方差(RMSECV)、校正均方差(RMSEC)分别为0.998 2,0.189 9,0.051 4.经外部验证,校正模型的预测均方差(RMSEP)、平均回收率分别为0.080 2,104.27%.结论:该方法准确、快速、简便,可实现大批量样品的快速分析.

Determination content of baicalin in Yinhuang granules from differentpharmaceutical factories by near-infrared spectroscopy

Objective : To determine the content of baicalin in Yinhuang granules fastly from different pharmaceutical factories with near-infrared spectroscopy. Method : Near-infrared diffuse reflectance spectroscopy technology was used to collect NIR spectra of Yinhuang granules.With HPLC anilysis values as reference,the fast determination method of baicalin was established with partial least squares(PLS). Result : The correlation coefficients (R²),the root-mean-square error of cross-validation and the root-mean-square error of calibration(RMSEC) of the calibration model for baicalin were 0.998 2,0.189 9 and 0.051 4, respectively;the root-mean-square error of prediction(RMSEP) and the average rate of recovery were 0.080 2 and 104.27%. Conclusion : The method is accurate,fast and simple,and can be applied for the fast determination of large quantities of numbers of samples.