| 甘草β-香树酯醇合成酶编码区SNP与甘草酸含量的相关性研究 |
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| 引用本文： | 沈湛云,刘春生,王学勇,呙未,李贝宁.甘草β-香树酯醇合成酶编码区SNP与甘草酸含量的相关性研究[J].中国中药杂志,2010,35(7):813-816. |
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| 作者姓名： | 沈湛云 刘春生 王学勇 呙未 李贝宁 |
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| 作者单位： | 北京中医药大学,中药学院,北京,100102 |
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| 基金项目： | 国家自然科学基金项目(30672615) |
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| 摘 要： | 目的:探讨甘草β-香树酯醇合成酶(β-amyrin synthase,bAS)编码区SNP与甘草酸含量之间的相关性。方法:采用HPLC法测量80株人工栽培甘草的甘草酸含量,采用SAS 9.0软件将80株甘草按照甘草酸含量极显著水平(P0.000 1)进行分组;采用RT-PCR技术,扩增出甘草bAS编码区序列,运用DNAman分析软件找出该序列的SNP位点,进而分析该位点与甘草酸含量高低的相关性。结果:bAS基因编码区共有94,254 bp 2个突变位点,在94 bp位点发生G/A转换,为错义突变,导致该位点处甘氨酸/天冬氨酸转换,254 bp处发生C/T转换,为同义突变,根据序列变异将所测样品划分成G-T基因型、A-T基因型、G-C基因型和A/G-C基因型。结论:A-T基因型、G/A-C基因型和G-T基因型和高含量甘草酸形成具有显著的相关性。
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| 关 键 词： | 甘草酸 β-香树酯醇合成酶 单核苷酸多态性 |
| 收稿时间： | 2009/12/7 0:00:00 |
Correlation analysis between single nucleotide polymorphism of β-amyrin synthase and content of glycyrrhizic acid in Glycyrrhiza uralensis |
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| SHEN Zhanyun,LIU Chunsheng,WANG Xueyong,GUO Wei and LI Beining.Correlation analysis between single nucleotide polymorphism of β-amyrin synthase and content of glycyrrhizic acid in Glycyrrhiza uralensis[J].China Journal of Chinese Materia Medica,2010,35(7):813-816. |
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| Authors: | SHEN Zhanyun LIU Chunsheng WANG Xueyong GUO Wei and LI Beining |
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| Institution: | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | |
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| Keywords: | glycyrrhizic acid β-amyrin synthase single nucleotide polymorphism |
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